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Titolo:
Endothelin-B-receptors-dependent-inhibition of platelet aggregation in theCD-1 mouse
Autore:
Labonte, J; Bkaily, G; DOrleans-Juste, PD;
Indirizzi:
Univ Sherbrooke, Sch Med, Inst Pharmacol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 erbrooke, PQ J1H 5N4, Canada Univ Sherbrooke, Sch Med, Dept Anat & Cell Biol, Sherbrooke, PQ J1H 5N4, Canada Univ Sherbrooke Sherbrooke PQ Canada J1H 5N4 erbrooke, PQ J1H 5N4, Canada
Titolo Testata:
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
, volume: 36, anno: 2000, supplemento:, 1
pagine: S184 - S186
SICI:
0160-2446(2000)36:<S184:EOPAIT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTACYCLIN;
Keywords:
endothelin (ET); mice; platelet aggregation; endothelin receptor; indomethacin; IRL-1620;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
6
Recensione:
Indirizzi per estratti:
Indirizzo: D'Orleans-Juste, PD Univ Sherbrooke, Sch Med, Inst Pharmacol, 3001,12 Ave Nord, Sherbrooke, PQJ1H 5N4, Canada Univ Sherbrooke 3001,12 Ave Nord Sherbrooke PQ Canada J1H 5N4
Citazione:
J. Labonte et al., "Endothelin-B-receptors-dependent-inhibition of platelet aggregation in theCD-1 mouse", J CARDIO PH, 36, 2000, pp. S184-S186

Abstract

An experimental protocol of adenosine diphosphate(ADP) induced platelet aggregation in the mouse was designed in order to study the roles played by endothelin-A (ETA) and endothelin-B (ETB) receptors in the ET-1-induced inhibition of ex vivo platelet aggregation. The pressor effects of ET-1 or IRL-1620 were firstly determined in vivo in anesthetized (ketamine/xylazine) CD-1 mice (males and females; 25-30 g). All agents were administered intravenously (via the jugular vein) and blood samples were collected from the carotid artery into heparinized Eppendorfs (15 U/ml). To obtain platelet-rich plasma (PRP) the blood was immediately centrifuged for 12 min at low speed (1100 g). Platelet-poor plasma (PPP) was then prepared by centrifugation of the whole blood sample at high speed (3700 g) for 30 min. PPP was used to calibrate the aggregometer at 100% transmission. Platelet aggregation was monitored ex vivo as a change in light transmission through PRP following theinjection of ADP (5 muM). ET-1 (0.01-2.0 nmol/kg) induced a significant and dose-dependent inhibition of platelet aggregation ex vivo (12-84%). The selective ETB agonist. IRL-1620 (0.05-2.0 nmol/kg), also triggered a marked inhibition of platelet aggregation. Indomethacin (10 mg/kg), a nonselectivecyclooxygenase inhibitor, abolished the inhibitory effect of ET-1. The selective ETA antagonist, BQ-123 (1 nmol/kg), abolished the in vivo presser effect of exogenous ET-1, without affecting its anti-aggregatory activity. The selective ETB antagonist, BQ-788 (0.5 nmol/kg), did not modify the elevation of blood pressure produced by the ET-1; however, it did abrogate dose-dependently the inhibitory effect of ET-1 on platelet aggregation. These results suggest that the anti-aggregatory effect of ET-1, in anesthetized CD-1mice, relies mainly upon the activation of ETB receptors. The mechanism whereby ET-1 exerts this effect, is partially indirect and requires at least the production and the release of prostanoids (possibly PGI(2)) into the blood stream.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:34:54