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Titolo:
Apolipoprotein E receptors mediate the effects of beta-amyloid on astrocyte cultures
Autore:
LaDu, MJ; Shah, JA; Reardon, CA; Getz, GS; Bu, G; Hu, JR; Guo, L; Van Eldik, LJ;
Indirizzi:
Northwestern Univ, Sch Med, NW Drug Discovery Program, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 ery Program, Chicago, IL 60611 USA Evanston NW Healthcare Res Inst, Dept Med, Evanston, IL 60201 USA EvanstonNW Healthcare Res Inst Evanston IL USA 60201 nston, IL 60201 USA Univ Chicago, Dept Pathol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 ago, Dept Pathol, Chicago, IL 60637 USA Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ St Louis MO USA 63110 l & Physiol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Dept Pediat, St Louis, MO 63110 USA Northwestern Univ, Dept Cell & Mol Biol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 & Mol Biol, Chicago, IL 60611 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 43, volume: 275, anno: 2000,
pagine: 33974 - 33980
SICI:
0021-9258(20001027)275:43<33974:AERMTE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NITRIC-OXIDE SYNTHASE; E MESSENGER-RNA; LIPOPROTEIN-RECEPTOR; ALZHEIMERS-DISEASE; PROTEIN LRP; CEREBROSPINAL-FLUID; PRECURSOR PROTEIN; 39-KDA PROTEIN; APOE RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Van Eldik, LJ Northwestern Univ, Sch Med, NW Drug Discovery Program, 303 EChicago Ave,Ward 4-202, Chicago, IL 60611 USA Northwestern Univ 303 E Chicago Ave,Ward 4-202 Chicago IL USA 60611
Citazione:
M.J. LaDu et al., "Apolipoprotein E receptors mediate the effects of beta-amyloid on astrocyte cultures", J BIOL CHEM, 275(43), 2000, pp. 33974-33980

Abstract

We have previously shown that beta -amyloid (A beta) induces astrocyte activation in vitro and that this reaction is attenuated by the addition of exogenous apolipoprotein E (apoE)-containing particles. However, the effects of A beta on endogenous apoE and apoJ levels and the potential role of apoEreceptors in astrocyte activation have not been addressed. Three activating stimuli (lipopolysaccharide, dibutyryl cAMP, and aged A beta 1-42) were used to induce activation of rat astrocyte cultures, as assessed by changes in morphology and an increase in interleubin-1 beta. However, only A beta also induced similar to 50% reduction in the amount of released apoE and apoJ and an 8-fold increase in the levels of cell-associated apoE and apoJ, Experiments using two concentrations of receptor-associated protein, an inhibitor of apoE receptors with a differential affinity for the low density lipoprotein receptor (LDLR) and the LDLR-related protein (LRP), suggest that LRP mediates A beta -induced astrocyte activation, whereas LDLR mediates theA beta -induced changes in apoE levels. Receptor-associated protein had noeffect on apoJ levels or on activation by either dibutyryl cAMP or lipopolysaccharide. These data suggest that apoE receptors translate the presence of extracellular A beta into cellular responses, both initiating and modulating the inflammatory response induced by A beta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:43:25