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Titolo:
Expression of a catalytically inactive H118Y mutant of nm23-H2 suppresses the metastatic potential of Line IVCl 1 human melanoma cells
Autore:
Hamby, CV; Abbi, R; Prasad, N; Stauffer, C; Thomson, J; Mendola, CE; Sidorov, V; Backer, JM;
Indirizzi:
New York Med Coll, Dept Microbiol & Immunol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 & Immunol, Valhalla, NY 10595 USA New York Med Coll, Dept Pathol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 pt Pathol, Valhalla, NY 10595 USA SibTech Inc, Newington, CT USA SibTech Inc Newington CT USASibTech Inc, Newington, CT USA
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 4, volume: 88, anno: 2000,
pagine: 547 - 553
SICI:
0020-7136(20001115)88:4<547:EOACIH>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOSIDE DIPHOSPHATE KINASE; IN-VITRO; GENES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Hamby, CV New York Med Coll, Dept Microbiol & Immunol, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 Valhalla, NY 10595 USA
Citazione:
C.V. Hamby et al., "Expression of a catalytically inactive H118Y mutant of nm23-H2 suppresses the metastatic potential of Line IVCl 1 human melanoma cells", INT J CANC, 88(4), 2000, pp. 547-553

Abstract

Nm23-H1 and nm23-H2 are putative metastasis suppressor genes that encode nucleoside diphosphate kinase (NDPK) A and B, NDPKs form oligomers distributed between soluble and particulate fractions of cells and therefore may exert their effects as either soluble or bound proteins. To determine whether metastasis-related functions of NDPKs are mediated by their catalytic activity in membrane bound or soluble complexes, we have stably transfected highly metastatic human melanoma Line IV CI I cells with wild-type and catalytically inactive (HI 18Y) nm23-H1 and nm23-H2 genes and assayed their metastatic potential in nude mice. Transfection with wild-type nm23-H1 and nm23-H2genes and catalytically inactive nm23-H1 did not significantly (all p > 0.10) alter the metastatic potential of Line IV CI cells while transfection with catalytically inactive nm23-H2 significantly (p < 0.01) reduced their metastatic potential. The lack of effect of transfection with wild-type and catalytically inactive nm23-H1 suggests that neither soluble nor membrane bound NDPK A affect the metastatic potential of Line IV CI I cells. The metastasis suppressive effect of catalytically inactive NDPK B overexpression suggests that competition with bound complexes containing catalytically active NDPK B inhibits metastasis of Line IV CI I cells, These results imply that bound NDPK B promotes metastasis and suggest that inhibition of its function or of its binding to critical sites may be a useful approach to limit the development of metastases in human melanoma, Int. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 11:12:16