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Titolo:
Cell mechanics studied by a reconstituted model tissue
Autore:
Wakatsuki, T; Kolodney, MS; Zahalak, GI; Elson, EL;
Indirizzi:
Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Mol Biophys, St Louis, MO 63110 USA Univ Calif Los Angeles, Sch Med, MacDonald Res Lab 1519, Dept Med,Div Dermatol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Washington Univ, Sch Engn, Dept Biomed Engn, St Louis, MO 63130 USA Washington Univ St Louis MO USA 63130 Biomed Engn, St Louis, MO 63130 USA Washington Univ, Sch Engn, Dept Mech Engn, St Louis, MO 63130 USA Washington Univ St Louis MO USA 63130 t Mech Engn, St Louis, MO 63130 USA
Titolo Testata:
BIOPHYSICAL JOURNAL
fascicolo: 5, volume: 79, anno: 2000,
pagine: 2353 - 2368
SICI:
0006-3495(200011)79:5<2353:CMSBAR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT-CHAIN; ISOMETRIC CONTRACTION; FIBROBLAST TRACTION; COLLAGEN LATTICES; 3T3 FIBROBLASTS; CALYCULIN-A; PHOSPHORYLATION; CYTOSKELETON; STRESS; FORCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Elson, EL Washington Univ, Sch Med, Dept Biochem & Mol Biophys, 600 S Euclid Ave,Box8231, St Louis, MO 63110 USA Washington Univ 600 S Euclid Ave,Box8231 St Louis MO USA 63110
Citazione:
T. Wakatsuki et al., "Cell mechanics studied by a reconstituted model tissue", BIOPHYS J, 79(5), 2000, pp. 2353-2368

Abstract

Tissue models reconstituted from cells and extracellular matrix (ECM) simulate natural tissues. Cytoskeletal and matrix proteins govern the force exerted by a tissue and its stiffness. Cells regulate cytoskeletal structure and remodel ECM to produce mechanical changes during tissue development and wound healing. Characterization and control of mechanical properties of reconstituted tissues are essential for tissue engineering applications. We have quantitatively characterized mechanical properties of connective tissue models, fibroblast-populated matrices (FPMs), via uniaxial stretch measurements. FPMs resemble natural tissues in their exponential dependence of stress on strain and linear dependence of stiffness on force at a given strain. Activating cellular contractile forces by calf serum and disrupting F-actin by cytochalasin D yield "active" and "passive" components, which respectively emphasize cellular and matrix mechanical contributions. The strain-dependent stress and elastic modulus of the active component were independent of cell density above a threshold density. The same quantities for the passive component increased with cell number due to compression and reorganization of the matrix by the cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 06:11:33