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Titolo:
Quantitative analysis, by ultrastructural in situ hybridization, of mitochondrial genomes and their expression in mid-gut and ovarian cells of a mutant strain of Drosophila subobscura
Autore:
Lecher, P; Petit, N; Le Goff, S; Alziari, S;
Indirizzi:
Univ Clermont Ferrand 2, CNRS, UMR 6547, Equipe Genome Mitochondrial, F-63177 Clermont Ferrand, France Univ Clermont Ferrand 2 Clermont Ferrand France F-63177 Ferrand, France
Titolo Testata:
BIOLOGY OF THE CELL
fascicolo: 5, volume: 92, anno: 2000,
pagine: 341 - 350
SICI:
0248-4900(200008)92:5<341:QABUIS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSITU HYBRIDIZATION; DELETION MUTANT; DNA; LOCALIZATION; TRANSCRIPTS; CONSEQUENCES; HETEROPLASMY; MYOPATHIES; SECTIONS; TISSUES;
Keywords:
Drosophila subobscura; mtDNA deletion; heteroplasmy; ovary ultrastructural in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Alziari, S Univ Clermont Ferrand 2, CNRS, UMR 6547, Equipe Genome Mitochondrial, F-63177 Clermont Ferrand, France Univ Clermont Ferrand 2 Clermont Ferrand France F-63177 rance
Citazione:
P. Lecher et al., "Quantitative analysis, by ultrastructural in situ hybridization, of mitochondrial genomes and their expression in mid-gut and ovarian cells of a mutant strain of Drosophila subobscura", BIO CELL, 92(5), 2000, pp. 341-350

Abstract

In the mitochondrial deletion mutant strain studied here, two types of DNAcoexist (heteroplasmy): intact mtDNA (15.9 kb) and mutant mtDNA (10.9 kb),which represents about 80% of the mitochondrial genomes in somatic tissues. The heteroplasmy level is lower in ovary (63%). Mutation is transmitted unchanged through generations. Quantitative analysis of in situ DNA hybridization demonstrated that for the 12SrDNA probe, of a gene outside the deletion, the mitochondrial DNA cellular content in the studied cells of the mutant strain is 1.5 times higher than in the wild-type strain. For the probe encoding Cyto b, a mitochondrial gene affected by the mutation, the ratios (mutant versus wild-type content) differ according to cell type: close to 0.4 in MGE cells and 0.7 in ovary cells. These values indicate heteroplasmic levels of about 72% in MGE cells and 50% in stage 10 oocytes, which is lower than that previously reported for stage 14 oocytes (60%) and embryos (69%). Analysis of in situ RNA hybridization showed that for the 12SrDNA probe,the transcript concentrations do not differ significantly between MGE cells and cells of germinal origin from the two strains. For the Cyto b probe, the mutant RNA/wild-type RNA ratios are lower in somatic cells than in stage 10 nurse cells and oocytes, but in each case less than expected. These studies indicate that the progressive heteroplasmy increase may be related tointense phases of mitochondria biogenesis and that different compensatory phenomena may exist. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:50:07