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Titolo:
Clusterin gene in rat Sertoli cells is regulated by a core-enhancer element
Autore:
Lymar, ES; Clark, AM; Reeves, R; Griswold, MD;
Indirizzi:
Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 Biosci, Pullman, WA 99164 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 5, volume: 63, anno: 2000,
pagine: 1341 - 1351
SICI:
0006-3363(200011)63:5<1341:CGIRSC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE LEUKEMIA-VIRUS; TRANSCRIPTION FACTOR FAMILY; CHROMATIN FUNCTION; INDUCIBLE ELEMENT; BINDING-PROTEINS; APOLIPOPROTEIN-J; POINT MUTATIONS; RECEPTOR-DELTA; FACTOR-I; PROMOTER;
Keywords:
Sertoli cells; testes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Griswold, MD Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 man, WA 99164 USA
Citazione:
E.S. Lymar et al., "Clusterin gene in rat Sertoli cells is regulated by a core-enhancer element", BIOL REPROD, 63(5), 2000, pp. 1341-1351

Abstract

Clusterin is a ubiquitous glycoprotein that is promiscuously expressed at a low basal level but can be highly induced by a variety of stress conditions. In contrast, in some secretory cells associated with tissue-fluid interfaces such as the Sertoli cells in the testis, clusterin demonstrates high constitutive expression. In this study, we address the mechanisms that regulate the constitutive expression of the clusterin gene by using primary cultures of immature rat Sertoli cells. We have identified a region of the ratclusterin gene promoter that activated transcription only in Sertoli cellsand that mapped between positions -426 and -311. Sequence analysis of thisregion revealed a high concentration of potential regulatory elements. Using gel-shift assays combined with hydroxyl radical footprinting, we identified the elements recognized by the Sertoli cell nuclear factors. Comparisonof the interactions with this region of the nuclear factors from differentcell types demonstrated that recognition of the core-enhancer element is specific for the Sertoli cells, and in vitro, the core region was recognizedby the transcription factor CBF. Transient transfections showed that a core enhancer is responsible for more than a half of the total promoter activity and is an essential element for the cell-specific activity of the Sertoli-specific region. In addition to the core enhancer, tandem Spl sites are also required for maximal activity of this region.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 17:43:01