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Titolo:
Phylogenetic characterization and in situ detection of a Cytophaga-Flexibacter-Bacteroides phylogroup bacterium in Tuber borchii Vittad, ectomycorrhizal mycelium
Autore:
Barbieri, E; Potenza, L; Rossi, I; Sisti, D; Giomaro, G; Rossetti, S; Beimfohr, C; Stocchi, V;
Indirizzi:
Univ Urbino, Giorgio Fornaini Inst Biochem, I-61029 Urbino, Italy Univ Urbino Urbino Italy I-61029 ini Inst Biochem, I-61029 Urbino, Italy Univ Urbino, Inst Bot, I-61029 Urbino, Italy Univ Urbino Urbino Italy I-61029 Urbino, Inst Bot, I-61029 Urbino, Italy CNR, Water Res Inst, I-00198 Rome, Italy CNR Rome Italy I-00198CNR, Water Res Inst, I-00198 Rome, Italy Vermicon AG, D-80992 Munich, Germany Vermicon AG Munich Germany D-80992Vermicon AG, D-80992 Munich, Germany
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 11, volume: 66, anno: 2000,
pagine: 5035 -
SICI:
0099-2240(200011)66:11<5035:PCAISD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
16S RIBOSOMAL-RNA; FLAVOBACTER-BACTEROIDES; NUCLEOTIDE-SEQUENCE; MICROBIAL DIVERSITY; ESCHERICHIA-COLI; FRUIT BODIES; PURE CULTURE; IDENTIFICATION; SOIL; AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Stocchi, V Univ Urbino, Giorgio Fornaini Inst Biochem, Via Saffi 2, I-61029 Urbino, Italy Univ Urbino Via Saffi 2 Urbino Italy I-61029 029 Urbino, Italy
Citazione:
E. Barbieri et al., "Phylogenetic characterization and in situ detection of a Cytophaga-Flexibacter-Bacteroides phylogroup bacterium in Tuber borchii Vittad, ectomycorrhizal mycelium", APPL ENVIR, 66(11), 2000, pp. 5035

Abstract

Mycorrhizal ascomycetous fungi are obligate ectosymbionts that colonize the roots of gymnosperms and angiosperms. In this paper we describe a straightforward approach in which a combination of morphological and molecular methods was used to survey the presence of potentially endo- and epiphytic bacteria associated with the ascomycetous ectomycorrhizal fungus Tuber borchiiVittad. Universal eubacterial primers specific for the 5' and 3' ends of the 16S rRNA gene (16S rDNA) were used for PCR amplification, direct sequencing, and phylogenetic analyses, The 16S rDNA was amplified directly from four pure cultures of T. borchii Vittad, mycelium, A nearly full-length sequence of the gene coding for the prokaryotic small-subunit rRNA was obtained from each T. borchii mycelium studied. The 16S rDNA sequences were almost identical (98 to 99% similarity), and phylogenetic analysis placed them in asingle unique rRNA branch belonging to the Cytophaga-Flexibacter-Bacteroides (CFB) phylogroup which had not been described previously. In situ detection of the CFB bacterium in the hyphal tissue of the fungus T. borchii was carried out by using 16S rRNA-targeted oligonucleotide probes for the eubacterial domain and the Cytophaga-Flexibacter phylum, as well as a probe specifically designed for the detection of this mycelium-associated bacterium. Fluorescent in situ hybridization showed that all three of the probes used bound to the mycelium tissue. This study provides the first direct visual evidence of a not-yet-cultured CFB bacterium associated with a mycorrhizal fungus of the genus Tuber.

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Documento generato il 04/12/20 alle ore 01:27:03