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Titolo:
Glial cell line-derived neurotrophic factor (GDNF) is a proliferation factor for rat C6 glioma cells: evidence from antisense experiments
Autore:
Wiesenhofer, B; Weis, C; Humpel, C;
Indirizzi:
Univ Innsbruck Hosp, Dept Psychiat, Lab Psychiat, A-6020 Innsbruck, Austria Univ Innsbruck Hosp Innsbruck Austria A-6020 , A-6020 Innsbruck, Austria
Titolo Testata:
ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT
fascicolo: 5, volume: 10, anno: 2000,
pagine: 311 - 321
SICI:
1087-2906(200010)10:5<311:GCLNF(>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; MEDIATED GENE-TRANSFER; FACTOR MESSENGER-RNA; HUMAN ASTROCYTES; NERVOUS-SYSTEM; GLIOBLASTOMA CELLS; IN-VITRO; BETA-S; EXPRESSION; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Humpel, C Univ Innsbruck Hosp, Dept Psychiat, Lab Psychiat, Anichstr 35, A-6020 Innsbruck, Austria Univ Innsbruck Hosp Anichstr 35 Innsbruck AustriaA-6020 ustria
Citazione:
B. Wiesenhofer et al., "Glial cell line-derived neurotrophic factor (GDNF) is a proliferation factor for rat C6 glioma cells: evidence from antisense experiments", ANTISENSE N, 10(5), 2000, pp. 311-321

Abstract

Growth factors play an important role in proliferation and differentiationof malignant brain gliomas in humans. Glial cell line-derived neurotrophicfactor (GDNF) has been shown recently to be highly expressed in human glioblastomas and in rat glial cell lines B49 and C6. The aim of the present study was to knockdown GDNF, its receptor GFR-alpha1, and the related family member persephin by using antisense oligonucleotides and to observe the effects on cell proliferation. To enhance cellular uptake into C6 glioma cells, 15-mer phosphorothioate oligonucleotides were complexed with the cationiclipid Lipofectamine(TM). The complex was applied for 3 x 12 hours to C6 glioma cells, and cells were allowed to recover for 24 hours after each transfection and then analyzed. This protocol markedly reduced GDNF and GFR-alpha1 protein levels in C6 glioma cells compared with control oligonucleotides, Knockdown of C6 cells with GDNF and GFR-alpha1 but not with persephin antisense oligonucleotides significantly decreased the number of C6 glioma cells and also inhibited the incorporation of bromodeoxyuridine as a sign of reduced DNA synthesis. In conclusion, it is shown that GDNF but not persephin is a potent proliferation factor for rat glioma cells. Knockdown of GDNF using antisense oligonucleotides complexed with lipids as carriers may be useful in gene therapeutic approaches in vitro and possibly also in vivo.

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Documento generato il 14/07/20 alle ore 09:06:26