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Titolo:
Simultaneous overexpression of enzymes of the lower part of glycolysis canenhance the fermentative capacity of Saccharomyces cerevisiae
Autore:
Smits, HP; Hauf, J; Muller, S; Hobley, TJ; Zimmermann, FK; Hahn-Hagerdal, B; Nielsen, J; Olsson, L;
Indirizzi:
Tech Univ Denmark, Dept Biotechnol, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark Tech Univ Denmark Lyngby Denmark DK-2800 echnol, DK-2800 Lyngby, Denmark Univ Lund, Dept Appl Microbiol, S-22100 Lund, Sweden Univ Lund Lund Sweden S-22100 Dept Appl Microbiol, S-22100 Lund, Sweden Tech Univ Darmstadt, Inst Genet, D-64287 Darmstadt, Germany Tech Univ Darmstadt Darmstadt Germany D-64287 D-64287 Darmstadt, Germany
Titolo Testata:
YEAST
fascicolo: 14, volume: 16, anno: 2000,
pagine: 1325 - 1334
SICI:
0749-503X(200010)16:14<1325:SOOEOT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATABOLITE INACTIVATION; HEXOSE TRANSPORTERS; ZYMOMONAS-MOBILIS; METABOLIC FLUXES; ESCHERICHIA-COLI; YEAST-CELLS; GLUCOSE; SYSTEM; FRUCTOSE-2,6-BISPHOSPHATE; PHOSPHOFRUCTOKINASE;
Keywords:
Saccharomyces cerevisiae; lower glycolysis; glycolytic flux; fermentative capacity; glyceraldehyde-3-phosphate dehydrogenase; phosphoglycerate kinase; pyruvate kinase; pyruvate decarboxylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Olsson, L Tech Univ Denmark, Dept Biotechnol, Ctr Proc Biotechnol, Bldg 223, DK-2800Lyngby, Denmark Tech Univ Denmark Bldg 223 Lyngby Denmark DK-2800 ngby, Denmark
Citazione:
H.P. Smits et al., "Simultaneous overexpression of enzymes of the lower part of glycolysis canenhance the fermentative capacity of Saccharomyces cerevisiae", YEAST, 16(14), 2000, pp. 1325-1334

Abstract

Recombinant S. cerevisiae strains, with elevated levels of the enzymes of lower glycolysis (glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate mutase, phosphoglycerate kinase, enolase, pyruvate kinase, pyruvate decarboxylase and alcohol dehydrogenase) were physiologically characterized, During growth on glucose the enzyme Levels in the recombinant strains (YHM4 and YHM7) were 1.1-3,4-fold higher than in the host strain (CEN.PK.K45), The recombinant strains were grown in aerobic or anaerobic batch cultures on glucose or a mixture of glucose and galactose. The specific ethanol production rates in the recombinant strains were the same as for the host strain andthe physiological behaviour of the recombinant strains and the host strainwas similar, When the cellular demand for ATP was increased by means of glucose pulses (final concentrations of 3.9 gn or 2.0 gn, respectively) to aerobic chemostat cultures maintained at a dilution rate of 0.08/ h, the specific carbon dioxide production rate (qCO(2)) of CEN.PK.K45 accelerated at 6x 10(-3) mmol/g/min(2) during the first 15 min, whereas during the same time period the qCO(2) of YHM7 accelerated twice as fast at 12 x 10(-3) mmol/g/min(2), indicating a higher fermentative capacity in the recombinant strain. Copyright (C) 2000 John Wiley & Sons, Ltd.

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Documento generato il 02/04/20 alle ore 06:37:03