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Titolo:
Adenomatous polyposis coli protein contains two nuclear export signals andshuttles between the nucleus and cytoplasm
Autore:
Neufeld, KL; Nix, DA; Bogerd, H; Kang, YB; Beckerle, MC; Cullen, BR; White, RL;
Indirizzi:
Univ Utah, Dept Oncol Sci, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 l Sci, Salt Lake City, UT 84112 USA Univ Utah, Dept Biol, Huntsman Canc Inst, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 Inst, Salt Lake City, UT 84112 USA Duke Univ, Med Ctr, Howard Hughes Med Inst, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 oward Hughes Med Inst, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Genet, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 , Med Ctr, Dept Genet, Durham, NC 27710 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 22, volume: 97, anno: 2000,
pagine: 12085 - 12090
SICI:
0027-8424(20001024)97:22<12085:APCPCT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR PROTEIN; BETA-CATENIN; CELL-MIGRATION; APC; PATHWAY; REV; MUTATIONS; BINDING; SITES; CRM1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Neufeld, KL Univ Utah, Dept Oncol Sci, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 Lake City, UT 84112 USA
Citazione:
K.L. Neufeld et al., "Adenomatous polyposis coli protein contains two nuclear export signals andshuttles between the nucleus and cytoplasm", P NAS US, 97(22), 2000, pp. 12085-12090

Abstract

Mutational inactivation of the adenomatous polyposis coil (APC) tumor suppressor initiates most hereditary and sporadic colon carcinomas. Although APC protein is located in both the cytoplasm and the nucleus, the protein domains required to maintain a predominantly cytoplasmic localization are unknown. Here, we demonstrate that nuclear export of APC is mediated by two intrinsic, leucine-rich, nuclear export signals (NESs) located near the amino terminus. Each NEs was able to induce the nuclear export of a fused carrierprotein. Both APC NESs were independently able to interact with the Crm1 nuclear export factor and substitute for the HIV-1 Rev NEs to mediate nuclear mRNA export. Both APC NESs functioned within the context of APC sequence:an amino-terminal APC peptide containing both NESs interacted with Crm1 and showed nuclear export in a heterokaryon nucleocytoplasmic shuttling assay. Also, mutation of both APC NESs resulted in the nuclear accumulation of the full-length, similar to 320-kDa APC protein, further establishing that the two intrinsic APC NESs are necessary for APC protein nuclear export. Moreover, endogenous APC accumulated in the nucleus of cells treated with the Crm1-specific nuclear export inhibitor leptomycin B, Together, these data indicate that APC is a nucleocytoplasmic shuttle protein whose predominantlycytoplasmic localization requires NES function and suggests that APC may be important for signaling between the nuclear and cytoplasmic compartments of epithelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:13:52