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Titolo:
Comparative genetic patterns of glioblastoma multiforme: Potential diagnostic tool for tumor classification
Autore:
Wiltshire, RN; Rasheed, BKA; Friedman, HS; Friedman, AH; Bigner, SH;
Indirizzi:
Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA Duke Univ Durham NCUSA 27710 Med Ctr, Dept Pathol, Durham, NC 27710 USA Duke Univ, Dept Surg, Durham, NC 27710 USA Duke Univ Durham NC USA 27710Duke Univ, Dept Surg, Durham, NC 27710 USA Duke Univ, Dept Pediat, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 uke Univ, Dept Pediat, Durham, NC 27710 USA
Titolo Testata:
NEURO-ONCOLOGY
fascicolo: 3, volume: 2, anno: 2000,
pagine: 164 - 173
SICI:
1522-8517(200007)2:3<164:CGPOGM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; IN-SITU HYBRIDIZATION; MALIGNANT HUMAN GLIOMAS; ACUTE LYMPHOBLASTIC-LEUKEMIA; BRAIN-TUMORS; CELL-LINES; SOLID TUMORS; CHROMOSOMAL-ABNORMALITIES; CYTOGENETIC ANALYSIS; CDK4 AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Bigner, SH Duke Univ, Med Ctr, Dept Pathol, POB 3712, Durham, NC 27710 USADuke Univ POB 3712 Durham NC USA 27710 12, Durham, NC 27710 USA
Citazione:
R.N. Wiltshire et al., "Comparative genetic patterns of glioblastoma multiforme: Potential diagnostic tool for tumor classification", NEURO-ONCOL, 2(3), 2000, pp. 164-173

Abstract

Cytogenetic and molecular genetic studies of glioblastoma multiforme (GBM)have shown that the most frequent alterations are gains of chromosome 7, losses of 9p loci and chromosome 10, and gene amplification, primarily of the epidermal growth factor receptor (EGFR) gene. Although this profile is potentially useful in distinguishing GEM from other tumor types, the techniques used tend to be labor intensive, and some can detect only gains or losses of genetic loci. Comparative genomic hybridization (CGH) is a powerful technique capable of identifying both gains and losses of DNA sequences. The present study compares the CGH evaluation of 22 GEM with classic cytogenetics, loss of heterozygosity by allelotyping, and gene amplification by Southern blot analysis to determine the reliability of CGH in the genetic characterization of GEM. The CGH and karyotypic data were consistent in showing gain of chromosome 7 accompanied by a loss of chromosome 10 as the most frequent abnormality, followed by a loss of 9p in 17 of 22 GEM cases. Loss of heterozygosity of chromosomes 10 (19/22) and 9p (9/22) loci confirmed the underrepresentation by CGH. Genomic amplifications were observed by CGH in 5 of the 10 cases where gene amplification was detected by Southern blot analysis. The data show that CGH is equally reliable, compared with the more established genetic methods, for recognizing the prominent genetic alterations associated with GEM and support its use as a plausible adjunct to glioma classification.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:42:43