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Titolo:
Clusterin protein diversity in the primate eye
Autore:
Wong, P; Pfeffer, BA; Bernstein, SL; Chambers, ML; Chader, GJ; Zakeri, ZF; Wu, YQ; Wilson, MR; Becerra, SP;
Indirizzi:
Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada Univ Alberta Edmonton AB Canada T6G 2E9 Sci, Edmonton, AB T6G 2E9, Canada Univ Alberta, Dept Ophthalmol, Edmonton, AB T6G 2E9, Canada Univ Alberta Edmonton AB Canada T6G 2E9 mol, Edmonton, AB T6G 2E9, Canada Univ Alberta, Dept Med Genet, Edmonton, AB T6G 2E9, Canada Univ Alberta Edmonton AB Canada T6G 2E9 net, Edmonton, AB T6G 2E9, Canada Bausch & Lomb Pharmaceut, Pearl River, NY USA Bausch & Lomb Pharmaceut Pearl River NY USA rmaceut, Pearl River, NY USA Univ Maryland, Dept Ophthalmol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Ophthalmol, Baltimore, MD 21201 USA Fdn Fighting Blindness Inc, Hunt Valley, MD USA Fdn Fighting Blindness Inc Hunt Valley MD USA s Inc, Hunt Valley, MD USA CUNY Queens Coll, New York, NY USA CUNY Queens Coll New York NY USACUNY Queens Coll, New York, NY USA NEI, Retinal Cell & Mol Biol Lab, NIH, Bethesda, MD 20892 USA NEI Bethesda MD USA 20892 ell & Mol Biol Lab, NIH, Bethesda, MD 20892 USA Univ Wollongong, Dept Biol Sci, Wollongong, NSW 2500, Australia Univ Wollongong Wollongong NSW Australia 2500 ongong, NSW 2500, Australia
Titolo Testata:
MOLECULAR VISION
fascicolo: 23, volume: 6, anno: 2000,
pagine: 184 - 191
SICI:
1090-0535(20001027)6:23<184:CPDITP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOLIPOPROTEIN-J EXPRESSION; RETINAL-PIGMENT EPITHELIUM; RAT VENTRAL PROSTATE; MOLECULAR CHARACTERIZATION; MULTIFUNCTIONAL PROTEIN; SULFATED GLYCOPROTEIN-2; RETINITIS-PIGMENTOSA; TRPM-2 CLUSTERIN; GENE; COMPLEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Wong, P Univ Alberta, Dept Biol Sci, G504 Biol Sci CTR, Edmonton, AB T6G 2E9, Canada Univ Alberta G504 Biol Sci CTR Edmonton AB Canada T6G 2E9 , Canada
Citazione:
P. Wong et al., "Clusterin protein diversity in the primate eye", MOL VIS, 6(23), 2000, pp. 184-191

Abstract

Purpose: The clusterin gene encodes a multi-functional protein that has been identified in different tissues, including a number of different eye tissues, primarily in the mouse and to a much lesser extent in humans. Clusterin has been implicated in a number of cellular processes such as lipid transport, membrane integrity, apoptosis, and neurodegeneration, all of which could be important to the biology of the eye. In the current communication, we provide data that confirms the expression of clusterin in a number of different human eye tissues and establishes the expression profile of this gene in monkey derived eye tissues. The issue that we sought to examine is whether a broad profile of clusterin expression in the eye is consistent in primates (monkey and human). Methods: The majority of our study was done using monkey eye tissues. Where possible, we have used human tissues in order to confirm published findings. Northern and western analysis was performed using tissues derived from monkey eyes. In situ hybridization and immunochemistry were carried out on human eye sections. Results: Clusterin mRNA is expressed in primate lens, cornea, limbus, sclera, orbital muscle, ciliary body, retina, RPE/ choroid, and RPE cells in culture. Western analysis revealed that two major groups of clusterin exist in the eye, a high molecular weight group (>100 kDa) and a second group consisting of at least five clusterin species that are all approximately 80 kDa. Analysis of conditioned media from RPE cells cultured on permeable supports suggests that different forms of clusterin display alternative patterns of secretion. Conclusions: Clusterin is expressed in a broad range of eye tissues in both human and monkey, suggesting that this is a characteristic feature in primates. We demonstrate for the first time that a diverse number of clusterinisoforms were observed in monkey eye tissues by western analysis. Meanwhile, the molecular size of clusterin mRNA detected in the array of tissues are identical in size, suggesting that the nature of the diversity in clusterin forms is due to post-translational modifications. In addition, new insights were made in defining clusterin expression in ciliary body, cornea, andthe retinal pigment epithelium.

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Documento generato il 31/10/20 alle ore 15:27:29