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Titolo:
Efficient transduction of nondividing cells by optimized feline immunodeficiency virus vectors
Autore:
Curran, MA; Kaiser, SM; Achacoso, PL; Nolan, GP;
Indirizzi:
Stanford Univ, Med Ctr, Program Immunol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ogram Immunol, Stanford, CA 94305 USA Stanford Univ, Med Ctr, Dept Mol Pharmacol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Mol Pharmacol, Stanford, CA 94305 USA Stanford Univ, Med Ctr, Dept Microbiol & Immunol, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 iol & Immunol, Stanford, CA 94305 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 1, volume: 1, anno: 2000,
pagine: 31 - 38
SICI:
1525-0016(200001)1:1<31:ETONCB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG TERMINAL REPEAT; GENE-TRANSFER; DEOXYURIDINE-TRIPHOSPHATASE; NUCLEAR-LOCALIZATION; NUCLEOTIDE-SEQUENCE; REV TRANSACTIVATION; LENTIVIRAL VECTORS; IN-VIVO; INFECTION; EXPRESSION;
Keywords:
FIV; retrovirus; vector; gene therapy; gene transfer; RNA transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Curran, MA Stanford Univ, Med Ctr, Program Immunol, 300 Pasteur Dr, Stanford, CA 94305 USA Stanford Univ 300 Pasteur Dr Stanford CA USA 94305 CA 94305 USA
Citazione:
M.A. Curran et al., "Efficient transduction of nondividing cells by optimized feline immunodeficiency virus vectors", MOL THER, 1(1), 2000, pp. 31-38

Abstract

Second- and third-generation three-plasmid vector systems, termed FELIX, were constructed from feline immunodeficiency virus (FIV). To enhance vectorproduction, the weak FIV long terminal repeat promoter was replaced with the human cytomegalovirus enhancer/promoter. To construct a minimal system in which Gag-Pot was the only viral protein present, the cytoplasmic transport element was used in place of the FIV Rev-PRE system to facilitate nuclear export of Gag-Pol and the transfer vector. Unconcentrated vector titers routinely exceeded 1 x 10(6) IU/mL for most constructs tested. Second- and optimized third-generation vectors were capable of efficiently infecting G(1)/S-and G(2)/M-arrested cells. FIV-based FELIX vectors transduced human dendritic cells, hepatocytes, and aortic smooth muscle with efficiencies similar to that of a control 3T3 cell line. All three of these primary cell types were transducible by both the second- and third-generation FELIX vectors,demonstrating that FIV Gag-Pot alone contains the determinants necessary for transduction of primary cells. In cross-packaging tests, we observed that HIV Gag-Pol does not substantially package FIV vectors; consequently, useof such vectors in human immunodeficiency virus-infected cells should not lead to efficient mobilization of the inserted gene. Thus, this FIV-based vector system offers high efficiency and stable delivery of genes to numerous nondividing and primary cell types, opening new avenues for biological inquiry into normal human cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:27:58