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Titolo:
Adenovirus-mediated inducible gene expression in vivo by a hybrid ecdysonereceptor
Autore:
Hoppe, UC; Marban, E; Johns, DC;
Indirizzi:
Johns Hopkins Univ, Inst Mol Cardiobiol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 iobiol, Baltimore, MD 21205 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 2, volume: 1, anno: 2000,
pagine: 159 - 164
SICI:
1525-0016(200002)1:2<159:AIGEIV>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOID-X-RECEPTOR; TRANSGENIC MICE; MAMMALIAN-CELLS; ULTRASPIRACLE; SUPPRESSION; SUPERFAMILY; SYSTEM; LINE; ECR;
Keywords:
ecdysone receptor; in vivo; adenovirus; inducible expression; gene transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Johns, DC Johns Hopkins Univ, Inst Mol Cardiobiol, Ross 844,720 Rutland Ave, Baltimore, MD 21205 USA Johns Hopkins Univ Ross 844,720 Rutland Ave Baltimore MD USA 21205
Citazione:
U.C. Hoppe et al., "Adenovirus-mediated inducible gene expression in vivo by a hybrid ecdysonereceptor", MOL THER, 1(2), 2000, pp. 159-164

Abstract

Precise control of transgene expression would markedly facilitate certain applications of gene therapy. To regulate expression of a transferred gene in response to an exogenous compound in vivo,we modified the ecdysone-responsive system. We combined the advantages of the Drosophila (DmEcR) and the Bombyx ecdysone receptor (BmEcR) by creating a chimeric Drosophila/Bombyx ecdysone receptor (DB-EcR) that preserved the ability to bind to the modified ecdysone promoter without exogenous retinoid X receptor (RXR). In cultured cells, DB-EcR effectively mediates ligand-dependent transactivation of a reporter gene at lower concentrations of the chemical ecdysone agonist CS-Ethan VgRXR (DmEcR + RXR). Transgene delivery in vivo was achieved by intramyocardial injection of recombinant adenovirus vectors in adult rats. Upon stimulation with GS-E, DB-EcR potently (>40-fold induction) activated gene expression in vivo while VgRXR was not induced. This hybrid ecdysone receptor represents an important new tool for in vivo transgene regulation with potentially diverse applications in somatic and germline transfer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:30:38