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Titolo:
Adeno-associated virus mediates long-term gene transfer and delivery of chondroprotective IL-4 to murine synovium
Autore:
Watanabe, S; Imagawa, T; Boivin, GP; Gao, GP; Wilson, JM; Hirsch, R;
Indirizzi:
Childrens Hosp, Med Ctr, William S Rowe Div Rheumatol, Cincinnati, OH 45229 USA Childrens Hosp Cincinnati OH USA 45229 heumatol, Cincinnati, OH 45229 USA Univ Cincinnati, Div Comparat Pathol, Cincinnati, OH 45229 USA Univ Cincinnati Cincinnati OH USA 45229 Pathol, Cincinnati, OH 45229 USA Univ Penn Hlth Syst, Inst Human Gene Therapy, Philadelphia, PA 19104 USA Univ Penn Hlth Syst Philadelphia PA USA 19104 Philadelphia, PA 19104 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 2, volume: 2, anno: 2000,
pagine: 147 -
SICI:
1525-0016(200008)2:2<147:AVMLGT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLAGEN-INDUCED ARTHRITIS; RECOMBINANT ADENOASSOCIATED VIRUS; RHEUMATOID-ARTHRITIS; VIRAL IL-10; MONOCLONAL-ANTIBODY; CELL-LINE; TRANSGENE EXPRESSION; NERVOUS-SYSTEM; RABBIT KNEES; AAV VECTOR;
Keywords:
arthritis; synoviocyte; gene transfer; gene therapy; IL-4; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Hirsch, R Childrens Hosp, Med Ctr, William S Rowe Div Rheumatol, PAV 2-129,3333 Burnet Ave, Cincinnati, OH 45229 USA Childrens Hosp PAV 2-129,3333 Burnet Ave Cincinnati OH USA 45229
Citazione:
S. Watanabe et al., "Adeno-associated virus mediates long-term gene transfer and delivery of chondroprotective IL-4 to murine synovium", MOL THER, 2(2), 2000, pp. 147

Abstract

Treatments for rheumatoid arthritis and other inflammatory arthropathies are often ineffective at preventing joint destruction. Long-term genetic modification of the cells lining the joint space (synoviocytes) in vivo represents a potential method for the treatment of these chronic conditions. However, a vector capable of efficiently transducing synoviocytes in vivo for apersistent period has not been available. The present report describes thegenetic modification of synoviocytes in vivo using recombinant adeno-associated virus. High-titer adeno-associated virus encoding the gene for Escherichia coil beta -galactosidase was injected into the knee joints of mice. Synovial tissues were then examined for beta -galactosidase transgene expression by in situ staining and by fluorometry. High-efficiency, persistent transgene expression was observed in the synovium with no evidence of vector-induced inflammation. Expression was observed for at least 7 months and washigher in arthritic than nonarthritic mice. Gene transfer of murine IL-4 to the joints of mice with collagen-induced arthritis led to detectable levels of IL-4 in the joint and protection from articular cartilage destruction. These data suggest that adeno-associated virus may be a useful vector forgene delivery to the synovium for the treatment of inflammatory arthropathies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/05/20 alle ore 03:53:48