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Titolo:
Presenilin 2 mutation does not influence expression and concentration of APP forms in human platelets
Autore:
Pastorino, L; Colciaghi, F; Marcon, G; Borroni, B; Cottini, E; Cattabeni, F; Padovani, A; Di Luca, M;
Indirizzi:
Univ Milan, Inst Pharmacol Sci, I-20133 Milan, Italy Univ Milan Milan Italy I-20133 Inst Pharmacol Sci, I-20133 Milan, Italy Univ Udine, Dept Neurol, I-33100 Udine, Italy Univ Udine Udine Italy I-33100 Udine, Dept Neurol, I-33100 Udine, Italy Univ Brescia, Dept Med Sci, Brescia, Italy Univ Brescia Brescia ItalyUniv Brescia, Dept Med Sci, Brescia, Italy
Titolo Testata:
MOLECULAR MEDICINE
fascicolo: 10, volume: 6, anno: 2000,
pagine: 816 - 824
SICI:
1076-1551(200010)6:10<816:P2MDNI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYLOID PRECURSOR PROTEIN; FAMILIAL ALZHEIMERS-DISEASE; ALPHA-SECRETASE CLEAVAGE; BETA-SECRETASE; NEURONAL APOPTOSIS; MESSENGER-RNA; GENE; ISOFORMS; MEGAKARYOCYTES; MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Di Luca, M Univ Milan, Inst Pharmacol Sci, Via Balzaretti 9, I-20133 Milan, Italy Univ Milan Via Balzaretti 9 Milan Italy I-20133 3 Milan, Italy
Citazione:
L. Pastorino et al., "Presenilin 2 mutation does not influence expression and concentration of APP forms in human platelets", MOL MED, 6(10), 2000, pp. 816-824

Abstract

Background: The pattern of platelet amyloid precursor protein (APP) forms is altered in sporadic Alzheimer's disease patients, compared with both control subjects and non-Alzheimer's disease-demented patients. The aims of this study were to evaluate in platelets of symptomatic and presymptomatic patients carrying the mutation Met239Val in presenilin 2 (PS2) whether: i) PSZ and presenilin 3 (PS1! were expressed in platelets: ii) an altered expression of different APP isoforms mRNAs could be related to the presence of the mutation; and iii) an abnormal pattern of APP forms was associated to themutation. Materials and Methods: Reverse transcription-polymerase chain reaction (RT-PCR) of APP isoforms, PSI and PS2 was performed on RNA extracted from platelets of three PS2 Met239Val mutated subjects, seven sporadic Alzheimer's patients and nine control subjects. The pattern of platelet APP forms at protein level was evaluated in the same population of subjects by means of Western blots analysis with specific antibody. Results: We found that PS1 and PS2 were expressed correctly in human platelets. When the relative amount of expression of mRNA coding for APP 771/751-695 was measured, a similar ratio of expression was found in PSZ-mutated subjects, compared with both sporadic Alzheimer's patients and to control subjects. Furthermore, when APP forms were evaluated in platelet homogenates by means of Western blots analysis with appropriate antibody, no differencewas found in the pattern of APP forms in presence of PS2 mutation in platelets, compared with control subjects. Conclusions: These results indicated that PS2 was ex-pressed in human platelets and that PS2 mutation did not affect APP forms pattern, thus, suggesting that in this peripheral cell the pathological effect of PSZ mutation might occur upstream of the amyloid cascade.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 23:43:50