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Titolo:
Specific frequencies of spontaneous Ca2+ transients upregulate GAD 67 transcripts in embryonic spinal neurons
Autore:
Watt, SD; Gu, XN; Smith, RD; Spitzer, NC;
Indirizzi:
Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 t Biol, La Jolla, CA 92093 USA Univ Calif San Diego, Ctr Genet Mol, La Jolla, CA 92093 USA Univ Calif SanDiego La Jolla CA USA 92093 et Mol, La Jolla, CA 92093 USA
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 4, volume: 16, anno: 2000,
pagine: 376 - 387
SICI:
1044-7431(200010)16:4<376:SFOSCT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMIC-ACID DECARBOXYLASE; HYDROXYLASE GENE-EXPRESSION; GABA(A) RECEPTOR SUBUNIT; MESSENGER-RNAS; TYROSINE-HYDROXYLASE; SYMPATHETIC NEURONS; ACETYLCHOLINE-RECEPTOR; DEVELOPMENTAL EXPRESSION; REGULATED EXPRESSION; DEPENDENT REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Watt, SD Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 a Jolla, CA 92093 USA
Citazione:
S.D. Watt et al., "Specific frequencies of spontaneous Ca2+ transients upregulate GAD 67 transcripts in embryonic spinal neurons", MOL CELL NE, 16(4), 2000, pp. 376-387

Abstract

Spontaneous Ca2+ transients expressed prior to synaptogenesis regulate thedevelopmental appearance of GABA in cultured Xenopus spinal neurons. We find that glutamic acid decarboxylase (GAD) immunoreactivity is also Ca2+-dependent and parallels the appearance of GABA. We show that. xGAD 67 transcripts first appear in the embryonic spinal cord during the period in which these Ca2+ spikes are generated, in a pattern that is temporally and spatially appropriate to account for differentiation of GABAergic interneurons. RNase protection and competitive quantitative RT-PCR demonstrate that transcript levels are approximately threefold greater when neurons are cultured in the presence of extracellular Ca2+ that permits generation of transients than when cultured in its absence. The frequency of spontaneous Ca2+ spikes plays a crucial role in the regulation of transcripts, since reimposition ofCa2+ transients at the frequency generated in cultured neurons rescues normal expression. We conclude that naturally occurring low frequencies of these Ca2+ transients regulate levels of xGAD 67 mRNA in differentiating neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 17:58:20