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Titolo:
The use of growth factors, gene therapy and tissue engineering to improve meniscal healing
Autore:
Kasemkijwattana, C; Menetrey, J; Goto, H; Niyibizi, C; Fu, FH; Huard, J;
Indirizzi:
Childrens Hosp Pittsburgh, Dept Orthopaed Surg, Growth & Dev Lab, Pittsburgh, PA 15213 USA Childrens Hosp Pittsburgh Pittsburgh PA USA 15213 ittsburgh, PA 15213 USA Univ Pittsburgh, Collagen Biochem Lab, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 hem Lab, Pittsburgh, PA 15213 USA Univ Pittsburgh, Dept Orthopaed Surg, Div Sports Med, Athlet Dept, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 et Dept, Pittsburgh, PA 15213 USA
Titolo Testata:
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
fascicolo: 1-2, volume: 13, anno: 2000,
pagine: 19 - 28
SICI:
0928-4931(20000901)13:1-2<19:TUOGFG>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVITRO; CULTURE; COLLAGEN; REPAIR; CELLS; KNEE; FIBROCHONDROCYTES; MICROVASCULATURE; ARTHRITIS; CHILDREN;
Keywords:
growth factors; gene therapy; tissue engineering; meniscal healing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Huard, J Childrens Hosp Pittsburgh, Dept Orthopaed Surg & Mol Genet & Biochem, Pittsburgh, PA 15213 USA Childrens Hosp Pittsburgh Pittsburgh PA USA 15213 , PA 15213 USA
Citazione:
C. Kasemkijwattana et al., "The use of growth factors, gene therapy and tissue engineering to improve meniscal healing", MAT SCI E C, 13(1-2), 2000, pp. 19-28

Abstract

The meniscus plays important roles in the knee joint, including load transmission at the tibiofemoral articulation, shock absorption, lubrication, and stabilization of the knee joint, though its healing capacity remains limited. Meniscal healing requires the proliferation of meniscal fibrochondrocytes from either an intrinsic source at the site of injury or an extrinsic source from the blood supply or synovium. We have characterized the effects of various doses of nine growth factors on the meniscal fibrochondrocyte proliferation and collagen and non-collagen synthesis, and identified epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha), basicfibroblast growth factor (bFGF) and platelet derived growth factor AB (PDGF-AB) as candidate molecules to improve meniscal healing. The direct administration of the human recombinant growth factor protein is likely to be limited by the short biological half-life of these proteins and the rapid clearance of the injected proteins. We have therefore evaluated the feasibilityof gene therapy and tissue engineering to deliver marker genes into the meniscus and found that direct and myoblast mediated ex vivo gene transfer can be used to deliver high levels and persistent expression of these growth factors into the injured meniscus. This study will help in the development of strategies to improve meniscal healing using new innovative technologiessuch as gene therapy approaches. (C) 2000 Elsevier Science S.A. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:57:32