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Titolo:
Use of FDA and flow cytometry to assess metabolic activity as an indicatorof nutrient status in phytoplankton
Autore:
Brookes, JD; Geary, SM; Ganf, GG; Burch, MD;
Indirizzi:
Univ Adelaide, Dept Environm Biol, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 ol, Adelaide, SA 5005, Australia CRC Water Qual & Treatment, Salisbury, SA 5108, Australia CRC Water Qual &Treatment Salisbury SA Australia 5108 SA 5108, Australia
Titolo Testata:
MARINE AND FRESHWATER RESEARCH
fascicolo: 8, volume: 51, anno: 2000,
pagine: 817 - 823
SICI:
1323-1650(2000)51:8<817:UOFAFC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHEMICAL CHARACTERIZATION; MARINE MICROALGAE; PHOTOSYNTHESIS; FLUORESCENCE; LIMITATION; FLUOROCHROMES; ENRICHMENT; ALGAL; WATER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Brookes, JD CRC Water Qual & Treatment, PMB 3, Salisbury, SA 5108, Australia CRC Water Qual & Treatment PMB 3 Salisbury SA Australia 5108 a
Citazione:
J.D. Brookes et al., "Use of FDA and flow cytometry to assess metabolic activity as an indicatorof nutrient status in phytoplankton", MAR FRESH R, 51(8), 2000, pp. 817-823

Abstract

This study reports the use of a technique to determine nutrient limitationof cultured and natural phytoplankton. The technique, an FDA-activity assay, which is usually used to assess cell viability, was used to measure metabolic activity in response to nutrient addition; the metabolic activity of phytoplankton was determined as the rate of hydrolysis of fluorescein diacetate (FDA), by intracellular esterases, to fluorescein, which was detected using a flow cytometer. Replacement of the limiting nutrient to nitrogen- or phosphorus-limited cultures and field populations resulted in an increasein metabolic activity that was detectable 24 h after nutrient addition. Byflow cytometry, the natural phytoplankton community can be divided into different taxonomic groups; the response of these to FDA could be determined individually to allow identification of the nutrients limiting each type ofphytoplankton. This would be more specific than the assessment of a whole-community response, which may mask subtle differences among taxa.

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Documento generato il 21/09/20 alle ore 19:19:14