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Titolo:
Fixation of transsynaptically transported WGA-HRP and fluorescent dyes used in combination
Autore:
Herzog, J; Kummel, H;
Indirizzi:
Univ Kiel, Dept Physiol, D-24098 Kiel, Germany Univ Kiel Kiel Germany D-24098 Kiel, Dept Physiol, D-24098 Kiel, Germany
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 2, volume: 101, anno: 2000,
pagine: 149 - 156
SICI:
0165-0270(20000915)101:2<149:FOTTWA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONJUGATED HORSERADISH-PEROXIDASE; TRANS-NEURONAL TRANSPORT; HERPES-SIMPLEX VIRUS; SPINODELTOIDEUS MOTONEURONS; TRANSNEURONAL TRANSPORT; SYNAPTIC ACTIVITY; SPINAL-CORD; CAT; ACROMIODELTOIDEUS; HISTOCHEMISTRY;
Keywords:
aldehyde fixation; fluorescent dyes; interneurones; motoneurones; perfusion; periodate-lysine-paraformaldehyde (PLP); transsynaptic transport; WGA-HRP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Kummel, H Univ Kiel, Dept Physiol, Olshausenstr 40, D-24098 Kiel, Germany Univ Kiel Olshausenstr 40 Kiel Germany D-24098 98 Kiel, Germany
Citazione:
J. Herzog e H. Kummel, "Fixation of transsynaptically transported WGA-HRP and fluorescent dyes used in combination", J NEUROSC M, 101(2), 2000, pp. 149-156

Abstract

The transsynaptic transport of WGA-HRP is a powerful tool for the identification of last order interneurones in the spinal cord, but differentiation of primarily labelled motoneurones from transsynaptically labelled interneurones can be difficult. We therefore combined the transsynaptic labelling of interneurones with WGA-HRP and the labelling of motoneurones with fluorescent dyes (fluorogold, red beads, fast blue, DAPI) by injecting a mixture of the tracers into the nervi mediani of rats. The influence of different fixatives on the simultaneous preservation of WGA-HRP and fluorescence is evaluated. It is shown that, of several perfusion protocols, only perfusion with paraformaldehyde in addition to 1.4% lysine and 0.23% periodate allowed simultaneous visualisation of transsynaptic WGA-HRP and fluorescent dyes. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:04:08