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Titolo:
Augmented expression of cardiotrophin-1 and its receptor component, gp130,in both left and right ventricles after myocardial infarction in the rat
Autore:
Aoyama, T; Takimoto, Y; Pennica, D; Inoue, R; Shinoda, E; Hattori, R; Yui, Y; Sasayama, S;
Indirizzi:
Kyoto Univ, Fac Med, Dept Cardiovasc Med, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 ovasc Med, Sakyo Ku, Kyoto 6068507, Japan Genentech Inc, Dept Mol Oncol, S San Francisco, CA 94080 USA Genentech Inc S San Francisco CA USA 94080 S San Francisco, CA 94080 USA
Titolo Testata:
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
fascicolo: 10, volume: 32, anno: 2000,
pagine: 1821 - 1830
SICI:
0022-2828(200010)32:10<1821:AEOCAI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARDIAC MYOCYTES; CELL HYPERTROPHY; INTERLEUKIN-6; APOPTOSIS; PATHWAY; CLONING; HEART; INVOLVEMENT; ACTIVATION; INHIBITION;
Keywords:
myocardial infarction; ventricular remodeling; cardiotrophin-1; gp130; cytokine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Aoyama, T Kyoto Univ, Fac Med, Dept Cardiovasc Med, Sakyo Ku, 54 Kawaracho, Kyoto 6068507, Japan Kyoto Univ 54 Kawaracho Kyoto Japan 6068507 yoto 6068507, Japan
Citazione:
T. Aoyama et al., "Augmented expression of cardiotrophin-1 and its receptor component, gp130,in both left and right ventricles after myocardial infarction in the rat", J MOL CEL C, 32(10), 2000, pp. 1821-1830

Abstract

Cardiotrophin-1 (CT-l) is a potent cytokine that stimulates the assembly of sarcomeric units in series in cardiomyocytes through gp130 signaling, resulting in myocardial cell hypertrophy. To clarify the role of CT-1 and the gp130-signaling pathway during ventricular remodeling after myocardial infarction, we examined the expression of CT-1 and gp130 in a rat model of myocardial infarction. At 1, 3, 7, 14, 28 and 56 days (n=12 for each group) after ligation of a coronary artery, tissue samples were obtained from infarcttissue, the ventricular septum and the right ventricle. All animals developed large myocardial infarctions, with infarct sizes ranging from 39.8% to 50.3%. Progressive left ventricular dilatation and inadequate hypertrophy of the surviving myocardium were confirmed by echocardiography. CT-1 and gp130 mRNA levels were determined by semiquantitative reverse transcription-polymerase chain reaction using 1 or 5 mug of total RNA followed by Southern blotting. The densitometric analysis of the Southern blots revealed a significant increase in CT-1 and gp130 mRNA levels (P<0.01) compared with those of the sham-operated rats at: 1, 3, 7, 14, 28 and 56 days post-infarct in the infarct area, the ventricular septum (non-infarcted area) and right ventricle. The protein levels of CT-1 and gp130, determined by Western blot analysis, were significantly increased (P<0.05) compared with those of sham-operated rats, peaked during the acute stage and declined thereafter in the three regions described above. Immunohistochemical staining showed that CT-1and gp130-immunoreactivities were detected in cardiomyocytes and fibroblast-like cells and that the intensity of staining was increased at 7 days post-infarct compared with that in sham-operated rats. An augmented CT-1 and gp130 system thus appears to play an important role during ventricular remodeling after myocardial infarction. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:52:44