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Titolo:
Cu(II) and Zn(II) complexes with hyaluronic acid and its sulphated derivative - Effect on the motility of vascular endothelial cells
Autore:
Barbucci, R; Magnani, A; Lamponi, S; Mitola, S; Ziche, M; Morbidelli, L; Bussolino, F;
Indirizzi:
Univ Siena, Dept Chem & Biosyst Sci & Technol, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 osyst Sci & Technol, I-53100 Siena, Italy Univ Turin, IRCC, Inst Canc Res & Treament, Dept Genet Biol & Biochem, I-10060 Turin, Italy Univ Turin Turin Italy I-10060 enet Biol & Biochem, I-10060 Turin, Italy Univ Florence, CIMMBA, I-50139 Florence, Italy Univ Florence Florence Italy I-50139 ce, CIMMBA, I-50139 Florence, Italy
Titolo Testata:
JOURNAL OF INORGANIC BIOCHEMISTRY
fascicolo: 4, volume: 81, anno: 2000,
pagine: 229 - 237
SICI:
0162-0134(20001001)81:4<229:CAZCWH>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; ANGIOGENESIS INVIVO; AQUEOUS-SOLUTION; COPPER IONS; HEPARIN; BINDING; CALCIUM; ANTICOAGULATION; PROLIFERATION; MOBILIZATION;
Keywords:
metal ion complexes; sulphated hyaluronic acid; Cu(II) and Zn(II) complexes; endothelial cells; adhesion; migration; chemotaxis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Barbucci, R Univ Siena, Dept Chem & Biosyst Sci & Technol, Pian dei Mantellini 44, I-53100 Siena, Italy Univ Siena Pian dei Mantellini 44 Siena Italy I-53100 , Italy
Citazione:
R. Barbucci et al., "Cu(II) and Zn(II) complexes with hyaluronic acid and its sulphated derivative - Effect on the motility of vascular endothelial cells", J INORG BIO, 81(4), 2000, pp. 229-237

Abstract

With the aim of improving the compatibility of biomaterials to be used forthe construction of cardiovascular prosthesis, we have designed bioactive macromolecules resulting from chemical modifications of hyaluronic acid (Hyal). The stability constants of Cu(II) and Zn(II) complexes with the sulphated derivative of hyaluronic acid (HyalS(3.5)) were evaluated. Two different complexes have been found for each metal ion, CuL, Cu(OH)(2)L and ZnL, Zn(OH)(2)L (L means the disaccharide unit of the ligands) in aqueous solutionat 37 degreesC. The dihydroxo Cu(II) complex was present in high percentage at pH=7.4. On the contrary, the Zn(II) ion was present with a relatively low percentage of both complexes. The ability to stimulate endothelial celladhesion and migration was evaluated for Hyal, HyalS(3.5) and their complexes with Cu(II) and Zn(II) ions. The results revealed that Hyal and [Cu(OH)(2)HyalS(3.5)]((4.5)-) induced cell adhesion, while [ZnHyalS(3.5)]((2.5)-) and [Zn(OH)(2)HyalS(3.5)]((4.5)-) inhibited the process. The chemotactic activity of increasing concentrations of the above complexes was also evaluated, demonstrating that [Cu(OH)(2)HyalS(3.5)]((4.5)-) complex at 1 muM concentration was the most active in inducing cell migration. These results havebeen also strengthened by analysing adherent cell migration in agarose. Inconclusion, sulphated hyaluronic acid coordinated to Cu(IT) seems to be a promising matrix molecule for the construction of cardiovascular prosthesis. (C) 2000 Elsevier Science S.A. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 06:18:19