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Titolo:
Preliminary assays to elucidate the structure of oxytetracycline's degradation products in sediments - Determination of natural tetracyclines by high-performance liquid chromatography-fast atom bombardment mass spectrometry
Autore:
Delepee, R; Maume, D; Le Bizec, B; Pouliquen, H;
Indirizzi:
Ecole Natl Vet, INRA, UMR, F-44307 Nantes 03, France Ecole Natl Vet Nantes France 03 et, INRA, UMR, F-44307 Nantes 03, France Ecole Natl Vet, LDH, LNR, Lab Natl Reference Facteurs Croissance, F-44307 Nantes, France Ecole Natl Vet Nantes France F-44307 Croissance, F-44307 Nantes, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 748, anno: 2000,
pagine: 369 - 381
SICI:
1387-2273(20001010)748:2<369:PATETS>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESIDUAL TETRACYCLINES; CHEMICAL-ANALYSIS; ELECTROSPRAY-IONIZATION; ULTRAVIOLET DETECTION; BOVINE-TISSUES; ANTIBIOTICS; IDENTIFICATION; IMPROVEMENT; CHLORTETRACYCLINE; PHASE;
Keywords:
oxytetracycline; tetracycline; chlortetracycline; mass spectrometry; LC-MS; frit FAB; particle beam;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Delepee, R Ecole Natl Vet, INRA, UMR, Atlanpole La Chantrerie,BP 40706, F-44307 Nantes 03, France Ecole Natl Vet Atlanpole La Chantrerie,BP 40706 Nantes France 03
Citazione:
R. Delepee et al., "Preliminary assays to elucidate the structure of oxytetracycline's degradation products in sediments - Determination of natural tetracyclines by high-performance liquid chromatography-fast atom bombardment mass spectrometry", J CHROMAT B, 748(2), 2000, pp. 369-381

Abstract

A very specific high-performance liquid chromatography-mass spectrometric method for the determination of natural tetracyclines was developed in order to characterise the degradation products of oxytetracycline in sediments. First, extraction used a clean up step with a Bond Elut Certify(R) LRC cartridge. A 3 mum Spherisorb(R) ODS1 column was then used with a methanol, acetonitrile and oxalic acid mobile phase gradient. Chromatographic resolution in these conditions was 3.31 between oxytetracycline and tetracycline. Two liquid chromatography-mass spectrometry methodologies based on a particlebeam and a frit fast atom bombardment interface were developed. In the first approach, ionisation was performed in the negative chemical mode using methane as reacting gas. In the other case, glycerol-thioglycerol mixture was used as matrix to ensure good sensitivity. MS-MS experiment was performedto determinate oxytetracycline fragmentation pattern in the perspective ofdegradation product study. (C) 2000 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 15:04:50