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Titolo:
Expression of antisense to integrin subunit beta(3) inhibits microvascularendothelial cell capillary tube formation in fibrin
Autore:
Dallabrida, SM; De Sousa, MA; Farrell, DH;
Indirizzi:
Oregon Hlth Sci Univ, Sch Dent, Dept Oral Mol Biol, Portland, OR 97201 USAOregon Hlth Sci Univ Portland OR USA 97201 l Biol, Portland, OR 97201 USA Penn State Univ, Coll Med, Dept Biochem & Mol Biol, Hershey, PA 17033 USA Penn State Univ Hershey PA USA 17033 em & Mol Biol, Hershey, PA 17033 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 41, volume: 275, anno: 2000,
pagine: 32281 - 32288
SICI:
0021-9258(20001013)275:41<32281:EOATIS>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARG-GLY-ASP; FIBROBLAST GROWTH-FACTOR; ALPHA-V INTEGRINS; ALPHA(V)BETA(3) INTEGRIN; GLANZMANN THROMBASTHENIA; ADHESION MOLECULES; BLOOD-VESSELS; GENE-THERAPY; ANGIOGENESIS; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Farrell, DH Oregon Hlth Sci Univ, Sch Dent, Dept Oral Mol Biol, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 land, OR 97201 USA
Citazione:
S.M. Dallabrida et al., "Expression of antisense to integrin subunit beta(3) inhibits microvascularendothelial cell capillary tube formation in fibrin", J BIOL CHEM, 275(41), 2000, pp. 32281-32288

Abstract

alpha(v)beta(3) antagonists are potent angiogenesis inhibitors, and several different classes of inhibitors have been developed, including monoclonalantibodies, synthetic peptides, and small organic molecules. However, eachclass of inhibitor works by the same principal, by blocking the binding ofligands to alpha(v)beta(3). In an effort to develop an alpha(v)beta(3) inhibitor that down-regulates the actual level of alpha(v)beta(3), we developed an antisense strategy to inhibit alpha(v)beta(3) expression in vitro. beta(3) antisense expressed in endothelial cells specifically down-regulated alpha(v)beta(3) and inhibited capillary tube formation, with the extent of down-regulation correlating with the extent of tube formation inhibition. This inhibition was matrix-specific, since tube formation was not inhibited in Matrigel. These findings support the notion that alpha(v)beta(3) is required for an essential step of angiogenesis in fibrin, namely capillary tube formation. These results suggest that pseudogenetic inhibition of beta(3) integrins using antisense techniques may ultimately provide a therapeutic means to inhibit angiogenesis in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 04:53:08