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Titolo:
Neisseria gonorrhoeae porin modifies the oxidative burst of human professional phagocytes
Autore:
Lorenzen, DR; Gunther, D; Pandit, J; Rudel, T; Brandt, E; Meyer, TF;
Indirizzi:
Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany Max Planck Inst Infekt Biol Berlin Germany D-10117 10117 Berlin, Germany Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany Max Planck Inst Biol Tubingen Germany D-72076 D-72076 Tubingen, Germany Forschungszentrum Borstel, Abt Immunol & Zellbiol, D-23845 Borstel, Germany Forschungszentrum Borstel Borstel Germany D-23845 23845 Borstel, Germany
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 11, volume: 68, anno: 2000,
pagine: 6215 - 6222
SICI:
0019-9567(200011)68:11<6215:NGPMTO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT CHEMI-LUMINESCENCE; HUMAN-NEUTROPHILS; EPITHELIAL-CELLS; OXYGEN METABOLITES; POLYMORPHONUCLEAR LEUKOCYTES; BACTERIAL LIPOPOLYSACCHARIDE; MEMBRANE-PROTEINS; ENHANCED RELEASE; DE-GRANULATION; MYELOPEROXIDASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, TF Max Planck Inst Infekt Biol, Mol Biol Abt, Schumannstr 21-22, D-10117 Berlin, Germany Max Planck Inst Infekt Biol Schumannstr 21-22 BerlinGermany D-10117
Citazione:
D.R. Lorenzen et al., "Neisseria gonorrhoeae porin modifies the oxidative burst of human professional phagocytes", INFEC IMMUN, 68(11), 2000, pp. 6215-6222

Abstract

A hallmark of infection with the gram-negative bacterium Neisseria gonorrhoeae is the local infiltration and subsequent activation of polymorphonuclear neutrophils, Several gonococcal outer membrane proteins are involved in the interaction with and the activation of these phagocytes, including gonococcal porin, the most abundant protein in the outer membrane. Previous work suggests that this porin plays a role in various cellular processes, including inhibiting neutrophils activation and phagosome maturation in professional phagocytes, Here we investigated the ability of porin to modify the oxidative metabolism of human peripheral blood neutrophils and monocytes in response to particulate stimuli (including live gonococci) and soluble agents. The activation of the oxidative metabolism was determined by chemiluminescence amplified with either luminol or Lucigenin. We found that treatmentof the phagocytes with porin inhibits the release of reactive oxygen species measured as luminol-enhanced chemiluminescence in response to zymosan, latex particles, and gonococci. The engulfment of these particles was not, however, affected by porin treatment. Similar effects of porin on the chemiluminescence response were observed in cytochalasin B-treated neutrophils exposed to the soluble chemotactic peptide N-formylmethionyl-leucyl-phenylalanine. This indicates that porin selectively inhibits granule fusion with those cellular membranes that are in direct contact with porin, namely, the phagosomal and plasma membranes, This porin-induced downregulation of oxidative metabolism may be a potent mechanism by which gonococci modulate oxygen-dependent reactions by activated phagocytes at inflammation sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:28:54