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Titolo:
INFLUENCE OF CARCINOGEN BINDING BY LACTIC ACID-PRODUCING BACTERIA ON TISSUE DISTRIBUTION AND IN-VIVO MUTAGENICITY OF DIETARY CARCINOGENS
Autore:
BOLOGNANI F; RUMNEY CJ; ROWLAND IR;
Indirizzi:
BRITISH IND BIOL RES ASSOC,DEPT MICROBIOL & NUTR,WOODMANSTERNE RD CARSHALTON SM5 4DS SURREY ENGLAND BRITISH IND BIOL RES ASSOC,DEPT MICROBIOL & NUTR CARSHALTON SM5 4DS SURREY ENGLAND
Titolo Testata:
Food and chemical toxicology
fascicolo: 6, volume: 35, anno: 1997,
pagine: 535 - 545
SICI:
0278-6915(1997)35:6<535:IOCBBL>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOST-MEDIATED ASSAY; GASTROINTESTINAL-TRACT; HETEROCYCLIC AMINES; INVITRO BINDING; RAT; GENOTOXICITY; ABSORPTION; FIBER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
F. Bolognani et al., "INFLUENCE OF CARCINOGEN BINDING BY LACTIC ACID-PRODUCING BACTERIA ON TISSUE DISTRIBUTION AND IN-VIVO MUTAGENICITY OF DIETARY CARCINOGENS", Food and chemical toxicology, 35(6), 1997, pp. 535-545

Abstract

The aims of this investigation were to determine whether viable cultures of lactic acid-producing organisms (LAB) can bind dietary carcinogens and to assess the consequences of binding for the absorption from the gut, distribution in the body and in vivo genotoxicity of ingestedcarcinogens. The carcinogens used in this study were ones known to bepresent in the human diet, namely benzo[a]pyrene (B(a)P, aflatoxin B-1 (AFB1) and the cooked food carcinogens 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3,8-dimethylimidazo[4,5-f]-quinoxaline (MeIQx), 5-phenyl-2-amino-1-methylimidazo [4,5-f]pyridine (PhIP) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). They represent a range of structural types so that the specificity of any binding effects could be addressed. Of the carcinogens tested, B(a)P and Trp-P-2 were bound most effectively by the two LAB strains Bifidobacterium longum and Lactobacillus acidophilus. AFB1 was poorly bound, while MeIQx, MeIQ, PhIP and IQ were bound to an intermediate degree. The extent of the binding of the heterocyclic amine carcinogens was dependent on the pH conditions during incubation and this effect was more apparent with B. longum than with L.acidophilus. Using the host-mediated assay (HMA), an in vivo bacterial mutation assay, it was demonstrated that the administration of bacterial cell suspensions of B. longum and L. acidophilus did not lead to a reduction in induced mutagenicity by MeIQ, MeIQx or Trp-P-2, detectable in the liver of treated mice compared with controls. The lack of aprotective effect could not be attributed to a short period of contact between bacterial cells and mutagens, since similar results were obtained after preincubating bacteria and mutagens together at pH 5 for 50-60 min, to maximize the binding, before gavaging the mice. Lack of activity of B(a)P in the HMA prevented the determination of the effect of LAB on genotoxicity of the polycyclic aromatic hydrocarbon. However, it is clear from the radiolabel distribution study that the amount of the carcinogen entering the blood was not significantly reduced by B. longum administration. In addition, the amount of radiolabelled B(a)P that reached the target organs (liver, lungs and heart) was also notaffected by the LAB administration. A similar lack of inhibitory effect of B. longum on blood concentration and accumulation in the liver of Trp-P-2 was apparent. The results of the present study suggest that although LAB may bind carcinogens in vitro, this does not lead to major changes in absorption and distribution of carcinogens in the body, or in their genotoxic activity in the liver. (C) 1997 Elsevier Science Ltd.

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Documento generato il 22/01/20 alle ore 21:44:38