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Titolo:
Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis
Autore:
Hernandez-Blazquez, FJ; Habib, M; Dumollard, JM; Barthelemy, C; Benchaib, M; de Capoa, A; Niveleau, A;
Indirizzi:
Univ Grenoble 1, UPRESA CNRS 5082, F-38706 La Tronche, France Univ Grenoble 1 La Tronche France F-38706 82, F-38706 La Tronche, France Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Pirassununga, SP, Brazil Univ Sao Paulo Pirassununga SP Brazil limentos, Pirassununga, SP, Brazil Univ Lyon 1, Fac Med, Ctr Commun Quantimetrie, F-69373 Lyon, France Univ Lyon 1 Lyon France F-69373 ommun Quantimetrie, F-69373 Lyon, France CHRU, Hop Bellevue, Serv Anat & Cytol Pathol, F-42055 St Etienne, France CHRU St Etienne France F-42055 Cytol Pathol, F-42055 St Etienne, France CHRU, Hop Nord, Serv Hepatol & Gastroenterol, F-42055 St Etienne, France CHRU St Etienne France F-42055 Gastroenterol, F-42055 St Etienne, France Univ Lyon 1, Fac Med, Reprod Biol Lab, F-69373 Lyon, France Univ Lyon 1 Lyon France F-69373 d, Reprod Biol Lab, F-69373 Lyon, France Univ Rome La Sapienza, Dipartimento Citogenet & Biol Mol, I-00161 Rome, Italy Univ Rome La Sapienza Rome Italy I-00161 & Biol Mol, I-00161 Rome, Italy
Titolo Testata:
GUT
fascicolo: 5, volume: 47, anno: 2000,
pagine: 689 - 693
SICI:
0017-5749(200011)47:5<689:EOGDHI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSISTED QUANTITATIVE-ANALYSIS; CHRONIC LYMPHOCYTIC-LEUKEMIA; TUMOR-SUPPRESSOR GENE; COLORECTAL-CANCER; MICROSATELLITE INSTABILITY; 5-METHYLCYTOSINE CONTENT; GENOMIC HYPOMETHYLATION; INTERPHASE NUCLEI; BREAST-CANCER; METHYLATION;
Keywords:
colon adenocarcinoma; DNA hypomethylation; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Niveleau, A Univ Grenoble 1, UPRESA CNRS 5082, Domaine Merci, F-38706 La Tronche, France Univ Grenoble 1 Domaine Merci La Tronche France F-38706 rance
Citazione:
F.J. Hernandez-Blazquez et al., "Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis", GUT, 47(5), 2000, pp. 689-693

Abstract

Background-Global hypomethylation of DNA is frequently observed in human tumours. This alteration is detected in early adenomas in colorectal tumorigenesis. Information is currently acquired after extraction of DNA from tissues, digestion with nucleases, and analysis by reverse phase chromatography, or treatment with restriction enzymes followed by gel electrophoresis analysis and Southern hybridisation with radiolabelled probes. Aims-The purpose of our work was to evaluate the global methylation statusof DNA in malignant lesions without loosing the histopathological featuresof the samples. Patients-The investigation was performed on paired normal-tumour tissues from 13 patients undergoing surgical resection of colorectal adenocarcinomas. Methods-Antibodies raised against 5-methylcytidine can be used to label methyl rich regions in interphase nuclei. This technique was adapted to the study of paraffin embedded tissues and an immunohistochemical method was developed to assess the global methylation status of individual nuclei while preserving cell morphology and tissue architecture. Computer assisted quantification of the staining intensity was performed on malignant and normal zones of human colon tissues to test the correlation between the immunolabelling signal and the respective histological patterns observed. Results-Qualitative and quantitative differences were observed and measured between the normal and malignant part of each sample. Morphologically altered nuclei displayed densely labelled spots within faintly labelled areas whereas normal nuclei were darker and uniformly stained. Image analysis allowed calculation of the average integrated optical density of the nuclei inboth types of tissues, demonstrating a constant and significantly lower intensity for the former type of cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/02/20 alle ore 17:19:51