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Titolo:
The membrane potential of Giardia intestinalis
Autore:
Biagini, GA; Lloyd, D; Kirk, K; Edwards, MR;
Indirizzi:
Univ Wales, Sch Biosci, Cardiff CF1 3TL, S Glam, Wales Univ Wales CardiffS Glam Wales CF1 3TL i, Cardiff CF1 3TL, S Glam, Wales Univ New S Wales, Sch Biochem & Mol Genet, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 t, Sydney, NSW 2052, Australia Australian Natl Univ, Fac Sci, Div Biochem & Mol Biol, Canberra, ACT 0200,Australia Australian Natl Univ Canberra ACT Australia 0200 erra, ACT 0200,Australia
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 1, volume: 192, anno: 2000,
pagine: 153 - 157
SICI:
0378-1097(20001101)192:1<153:TMPOGI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
H+-ATPASE; TRYPANOSOMA-CRUZI; CYTOPLASMIC PH;
Keywords:
anaerobic protozoa; bioenergetics; HC pumping; K+ diffusion pathway;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Lloyd, D Univ Wales, Sch Biosci, Cardiff CF1 3TL, S Glam, Wales Univ Wales Cardiff S Glam Wales CF1 3TL f CF1 3TL, S Glam, Wales
Citazione:
G.A. Biagini et al., "The membrane potential of Giardia intestinalis", FEMS MICROB, 192(1), 2000, pp. 153-157

Abstract

Giardia intestinalis is a primitive microaerophilic protozoan parasite which colonises the upper intestine of humans. Despite the evolutionary and medical significance of this organism, its physiology is very poorly understood. In this study we have used a novel flow cytometric technique to make quantitative measurements of the electrical potential across the plasma membrane of G. intestinalis trophozoites. In media lacking both K+ and Na+, G, intestinalis trophozoites maintained a high negative plasma membrane potential(Psi (m)) of -134 +/- 3 mV. The Psi (m) was unaffected by the addition ofNa+ to the extracellular medium, whereas the addition of K+ resulted in a significant membrane depolarisation, consistent with the G. intestinalis trophozoite plasma membrane having a significant (electrophoretic) permeability to K+. The membrane was also depolarised by the H+ ionophore m-chlorophenylhydrazone and by the H+ ATPase inhibitors dicyclohexylcarbodiimide and N-ethylmaleimide. These results are consistent with G. intestinalis trophozoites maintaining a high resting Psi (m), originating at least in part from an electrogenic H+ pump acting in concert with a K+ diffusion pathway. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:14:34