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Titolo:
Structural variation in the 16S-23S rRNA intergenic spacers of Vibrio parahaemolyticus
Autore:
Maeda, T; Takada, N; Furushita, M; Shiba, T;
Indirizzi:
Natl Fisheries Univ, Dept Food Sci & Technol, Yamaguchi 7596595, Japan Natl Fisheries Univ Yamaguchi Japan 7596595 ol, Yamaguchi 7596595, Japan
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 1, volume: 192, anno: 2000,
pagine: 73 - 77
SICI:
0378-1097(20001101)192:1<73:SVIT1R>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENE; ESCHERICHIA-COLI; MARINE BACTERIUM;
Keywords:
16S-23S rRNA; intergenic spacer; Vibrio parahaemolyticus; spacer tRNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Maeda, T Natl Fisheries Univ, Dept Food Sci & Technol, Yamaguchi 7596595, Japan Natl Fisheries Univ Yamaguchi Japan 7596595 uchi 7596595, Japan
Citazione:
T. Maeda et al., "Structural variation in the 16S-23S rRNA intergenic spacers of Vibrio parahaemolyticus", FEMS MICROB, 192(1), 2000, pp. 73-77

Abstract

The 16S-23S rRNA intergenic spacers (IGS) of Vibrio parahaemolyticus were PCR-amplified and cloned with pT7Blue T vector. A total of six clones were isolated dependent on size difference. The clones were different with respect to both the number and the composition of the tRNA genes included, and were designated IGS-0, IGS-E, IGS-IA, IGS-AE, IGS-EKV and IGS-EKAV. IGS-EKAVincluded the cluster of tRNA(Glu)-tRNA(Lys)-tRNA(Ala)-tRNA(Val); IGS-EKV, tRNA(Glu)-tRNA(Lys)-tRNA(Val); IGS-AE, tRNA(Ala)-tRNA(Glu); IGS-IA, tRNA(Ile)- tRNA(Ala); and IGS-E, tRNA(Glu). IGS-0 had no tRNA gene. Some similarities were found in the nucleotide sequence of the non-coding regions flankedby the tRNA genes. The structure difference found in the spacers is meaningful for elucidating the evolutionary line of each ribosomal RNA operon andthe profile is applicable as a molecular marker of the bacterium. (C) 2000Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:18:04