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Titolo:
Evidence for the dual coupling of the rat neurotensin receptor with pertussis toxin-sensitive and insensitive G-proteins
Autore:
Gailly, P; Najimi, M; Hermans, E;
Indirizzi:
Univ Catholique Louvain, Dept Physiol & Pharmacol, FARL, Lab Pharmacol Expt, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Univ Catholique Louvain, Dept Physiol & Pharmacol, FYMU, Lab Physiol Gen Muscles, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels BelgiumB-1200 B-1200 Brussels, Belgium
Titolo Testata:
FEBS LETTERS
fascicolo: 2-3, volume: 483, anno: 2000,
pagine: 109 - 113
SICI:
0014-5793(20001020)483:2-3<109:EFTDCO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOSOLIC PHOSPHOLIPASE A(2); GUANINE-NUCLEOTIDE-BINDING; HAMSTER OVARY CELLS; AGONIST-INDUCED INTERNALIZATION; ARACHIDONIC-ACID; CHO CELLS; GUANOSINE-5'-O-(3-THIO)TRIPHOSPHATE BINDING; ASPARTATE RESIDUE; BRAIN MEMBRANES; C ACTIVATION;
Keywords:
neurotensin; pertussis toxin; arachidonic acid; G-protein; GTP gamma S; phospholipase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Hermans, E Univ Catholique Louvain, Dept Physiol & Pharmacol, FARL, Lab Pharmacol Expt, Ave Hippocrate 54, B-1200 Brussels, Belgium Univ Catholique Louvain Ave Hippocrate 54 Brussels Belgium B-1200
Citazione:
P. Gailly et al., "Evidence for the dual coupling of the rat neurotensin receptor with pertussis toxin-sensitive and insensitive G-proteins", FEBS LETTER, 483(2-3), 2000, pp. 109-113

Abstract

We previously demonstrated the functional coupling of the rat neurotensin receptor NTS1 with G-proteins on transfected CHO cell homogenates by showing modulation of agonist affinity by guanylyl nucleotides and agonist-mediated stimulation of [S-35]GTP gammaS binding, In the present study, we observed that G(i/0)-type G-protein inactivation by pertussis toxin (PTx) resulted in a dramatic reduction of the NT-induced [S-35]GTP gammaS binding whereas the effect of guanylyl nucleotide was almost not affected, As expected, NT-mediated phosphoinositide hydrolysis and intracellular calcium mobilization were not altered after PTx treatment. This suggests the existence of multiple signaling cascades activated by NT, Accordingly, using PTx and the PLC inhibitor U-73122, we showed that both signaling pathways contribute to the NT-mediated production of arachidonic acid, These results support evidence for a dual coupling of the NTS1 with PTx-sensitive and insensitive G-proteins, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 06:52:22