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Titolo:
Inhibition of aflatoxin B1 genotoxicity in human liver-derived HepG2 cellsby kolaviron biflavonoids and molecular mechanisms of action
Autore:
Nwankwo, JO; Tahnteng, JG; Emerole, GO;
Indirizzi:
Univ Iowa, Coll Med, Dept Biochem, Iowa City, IA 52242 USA Univ Iowa IowaCity IA USA 52242 d, Dept Biochem, Iowa City, IA 52242 USA Univ Ibadan, Coll Med, Dept Biochem, Ibadan, Nigeria Univ Ibadan Ibadan Nigeria dan, Coll Med, Dept Biochem, Ibadan, Nigeria
Titolo Testata:
EUROPEAN JOURNAL OF CANCER PREVENTION
fascicolo: 5, volume: 9, anno: 2000,
pagine: 351 - 361
SICI:
0959-8278(200010)9:5<351:IOABGI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE-S-TRANSFERASE; UNSCHEDULED DNA-SYNTHESIS; GARCINIA-KOLA SEEDS; INDUCED HEPATOTOXICITY; METABOLIC-ACTIVATION; CYTOCHROMES P450; RAT; EXPRESSION; BINDING; CANCER;
Keywords:
aflatoxin B1; chemoprevention; cytochrome P450 3A4; genotoxicity; glutathione S-transferases; HepG2; kolaviron;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Nwankwo, JO Univ Iowa, Coll Med, Dept Biochem, BSB 4403, Iowa City, IA 52242 USA Univ Iowa BSB 4403 Iowa City IA USA 52242 a City, IA 52242 USA
Citazione:
J.O. Nwankwo et al., "Inhibition of aflatoxin B1 genotoxicity in human liver-derived HepG2 cellsby kolaviron biflavonoids and molecular mechanisms of action", EUR J CAN P, 9(5), 2000, pp. 351-361

Abstract

Kolaviron biflavonoids have demonstrated antihepatotoxic activity in animal studies. The present study investigated the possible chemopreventive potential of kolaviron in inhibiting aflatoxin B1 (AFB1) genotoxicity in HepG2 cells. Kolaviron inhibition of AFB1-induced cytotoxicity by clonogenic assay and genotoxicity by [H-3]thymidine incorporation in unscheduled DNA synthesis were evaluated, including antioxidant potential of kolaviron determined by its reduction in the intracellular reactive oxygen species level induced by hydrogen peroxide, Induction of AFB1-detoxicating enzymes such as cytochrome P450 3A4 (3A4) and glutathione S-transferases (GSTs) A1-1/ A2-2 (alpha) and M1B (mu) was determined by reverse transcription polymerase chain reaction (RT-PCR) and northern blotting for the messages and western immunoblot analysis for protein. Kolaviron significantly (P < 0.01) and dose-dependently inhibited the cytotoxicity (by 71.6%) and genotoxicity (47.1%) of AFB1 in HepG2 cells. The antioxidant potential of kolaviron compared favourably with values for the standard antioxidant trolox C (53.8% at only 4.5 x 10(-2)-fold kolaviron concentration) but was below that of butylated hydroxyanisole (58.1% at a ninefold kolaviron concentration). It induced about threefold increases in the messages for 3A4 and GSTs <alpha> and mu, including a twofold increase in GST alpha protein. Kolaviron may have chemopreventive potential in inhibition of human AFB1 genotoxicity and possibly hepatocarcinogenesis. (C) 2000 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:59:46