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Titolo:
Direct radiolabeling by [H-3]quisqualic acid of group I metabotropic glutamate receptor in rat brain synaptic membranes
Autore:
Hinoi, E; Ogita, K; Takeuchi, Y; Ohashi, H; Maruyama, T; Yoneda, Y;
Indirizzi:
Kanazawa Univ, Fac Pharmaceut Sci, Dept Mol Pharmacol, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9200934 wa, Ishikawa 9200934, Japan Banyu Pharmaceut Co Ltd, Tsukuba, Ibaraki 3002611, Japan Banyu Pharmaceut Co Ltd Tsukuba Ibaraki Japan 3002611 raki 3002611, Japan Setsunan Univ, Fac Pharmaceut Sci, Dept Pharmacol, Osaka 5730101, Japan Setsunan Univ Osaka Japan 5730101 , Dept Pharmacol, Osaka 5730101, Japan
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 881, anno: 2000,
pagine: 199 - 203
SICI:
0006-8993(20001027)881:2<199:DRB[AO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM DEPRESSION; MGLUR1 MUTANT MICE; MOLECULAR CHARACTERIZATION; BINDING; AGONIST; RADIOLIGAND; NEURONS; POTENT;
Keywords:
metabotropic glutamate receptor; [H-3]QA binding; group I agonists; GTP; scatchard analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Yoneda, Y Kanazawa Univ, Fac Pharmaceut Sci, Dept Mol Pharmacol, 13-1 Takara Machi, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ 13-1 Takara MachiKanazawa Ishikawa Japan 9200934
Citazione:
E. Hinoi et al., "Direct radiolabeling by [H-3]quisqualic acid of group I metabotropic glutamate receptor in rat brain synaptic membranes", BRAIN RES, 881(2), 2000, pp. 199-203

Abstract

[H-3]Quisqualic acid (QA) was synthesized and used to label metabotropic glutamate receptor (mGluR) in rat brain synaptic membranes in the presence of three different ionotropic glutamate receptor agonists at respective saturating concentrations. Of several mGluR agonists tested, group I agonists were more potent in displacing [H-3]QA binding than group II and group III agonists in the presence of the three ionotropic agonists. [H-3]QA binding was markedly inhibited by guanine nucleotide analogues in a concentration-dependent manner at a concentration range of 10 nM to 1 mM. Scatchard analysis revealed that [H-3]QA binding consisted of a single component with a K-d of 50.9+/-5.3 nM and a B-max of 431.6+/-18.3 fmol/mg protein. These resultssuggest that [H-3]QA indeed labels group I mGluR functionally coupled to GTP binding protein in rat brain synaptic membranes when determined under the experimental conditions employed. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 18:09:22