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Titolo:
Prevention of 1-methyl-4-phenylpyridinium- and 6-hydroxydopamine-induced nitration of tyrosine hydroxylase and neurotoxicity by EUK-134, a superoxidedismutase and catalase mimetic, in cultured dopaminergic neurons
Autore:
Pong, K; Doctrow, SR; Baudry, M;
Indirizzi:
Univ So Calif, Neurosci Program, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Program, Los Angeles, CA 90089 USA Eukar Inc, Bedford, MA USA Eukar Inc Bedford MA USAEukar Inc, Bedford, MA USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 2, volume: 881, anno: 2000,
pagine: 182 - 189
SICI:
0006-8993(20001027)881:2<182:PO1A6N>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; MITOCHONDRIAL COMPLEX-I; PARKINSONS-DISEASE; TRANSGENIC MICE; CELL-DEATH; DISTINCT MECHANISMS; LIPID-PEROXIDATION; OXIDATIVE DAMAGE; BRAIN INJURY; L-ARGININE;
Keywords:
catalase; dopaminergic neurons; oxidative stress; Parkinson's disease; reactive oxygen species; superoxide dismutase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Baudry, M Univ So Calif, Neurosci Program, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 os Angeles, CA 90089 USA
Citazione:
K. Pong et al., "Prevention of 1-methyl-4-phenylpyridinium- and 6-hydroxydopamine-induced nitration of tyrosine hydroxylase and neurotoxicity by EUK-134, a superoxidedismutase and catalase mimetic, in cultured dopaminergic neurons", BRAIN RES, 881(2), 2000, pp. 182-189

Abstract

Oxidative stress has been implicated in the selective degeneration of dopaminergic (DAergic) neurons in Parkinson's disease (PD). In this study, we tested the efficacy of EUK-134, a superoxide dismutase (SOD) and catalase mimetic, on the nitration of tyrosine hydroxylase (TH), a marker of oxidativestress, and neurotoxicity produced by 1-methyl-4-phenylpyridinium (MPP+) and 6-hydroxydopamine (6-OHDA) in primary DAergic neuron cultures. Exposure of cultures to 10 muM MPP+ reduced dopamine (DA) uptake and the number of tyrosine hydroxylase immunoreactive (THir) neurons to 56 and 52% of control,while exposure to 30 muM 6-OHDA reduced DA uptake and the number of THir neurons to 58 and 59% of control, respectively. Pretreatment of cultures with 0.5 muM EUK-134 completely protected DAergic neurons against MPP+- and 6-OHDA-induced neurotoxicity. Exposure of primary neuron cultures to either MPP+ or 6-OHDA produced nitration of tyrosine residues in TH. Pretreatment of cultures with 0.5 muM EUK-134 completely prevented MPP+- or 6-OHDA-induced nitration of tyrosine residues in TH. Taken together, these results support the idea that reactive oxygen species (ROS) are critically involved in MPP+- and 6-OHDA-induced neurotoxicity and suggest a potential therapeutic role for synthetic catalytic scavengers of ROS, such as EUK-134, in the treatment of PD. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:27:35