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Titolo:
Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering
Autore:
Roche, S; Ronziere, MC; Herbage, D; Freyria, AM;
Indirizzi:
CNRS UPR 412, Inst Biol & Chim Prot, F-69007 Lyon, France CNRS UPR 412 Lyon France F-69007 Biol & Chim Prot, F-69007 Lyon, France
Titolo Testata:
BIOMATERIALS
fascicolo: 1, volume: 22, anno: 2001,
pagine: 9 - 18
SICI:
0142-9612(200101)22:1<9:NADCCS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNTHETIC BIODEGRADABLE POLYMERS; ARTICULAR-CARTILAGE; IN-VITRO; BIOREACTOR CULTIVATION; HIGH-DENSITY; DEFECTS; REPAIR; CULTURE; MATRIX; IMPLANTS;
Keywords:
articular cartilage repair; collagen-based biomaterial; collagen synthesis; glycosaminoglycan deposition; mineral deposition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Freyria, AM CNRS UPR 412, Inst Biol & Chim Prot, 7 Passage Vercors, F-69007 Lyon, France CNRS UPR 412 7 Passage Vercors Lyon France F-69007 on, France
Citazione:
S. Roche et al., "Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering", BIOMATERIAL, 22(1), 2001, pp. 9-18

Abstract

Collagen-based biomaterials in the form of sponges (bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively) were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage. Control and DPPA sponges were initially seeded with 10(6) or 10(7) foetal bovine epiphyseal chondrocytes and maintained for 4 weeks in culture under static conditions in RPMI/NCTC mediumwith 10% FCS and without addition of fresh ascorbic acid. Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded. Cell proliferation was only noted in the 10(6) cells-seeded sponges (4-fold increase after 4 weeks of culture). Specific cartilage collagens (types II and XI) were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after 2-3 weeks and 4 weeks in the case of 10(6) and 10(7) cells-seeded sponges, respectively. Glycosaminoglycans accumulated in the matrix, up to 1.8 and 9.8% of total dry weight after one month with both seeding conditions, which was much lower thanin the natural tissue. In the 10(7) cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased (2- to 3-fold). These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. (C) 2000 Elsevier ScienceLtd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:47:17