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Titolo:
Donor origin of circulating endothelial progenitors after allogeneic bone marrow transplantation
Autore:
Ikpeazu, C; Davidson, MK; Halteman, D; Goodman, SA; Browning, PJ; Brandt, SJ;
Indirizzi:
Vanderbilt Univ, Med Ctr, Div Hematol Oncol, Bone Marrow Transplant Program, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 t Program, Nashville, TN 37232 USA Vanderbilt Univ, Med Ctr, Dept Med, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Dept Med, Nashville, TN 37232 USA Vanderbilt Univ, Med Ctr, Dept Cell Biol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Cell Biol, Nashville, TN 37232 USA Vanderbilt Univ, Med Ctr, Vanderbilt Ingram Canc Ctr, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Canc Ctr, Nashville, TN 37232 USA Nashville Vet Affairs Med Ctr, Nashville, TN USA Nashville Vet Affairs MedCtr Nashville TN USA ed Ctr, Nashville, TN USA
Titolo Testata:
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
fascicolo: 3A, volume: 6, anno: 2000,
pagine: 301 - 308
SICI:
1083-8791(2000)6:3A<301:DOOCEP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESENCHYMAL STEM-CELLS; PROCESSED TISSUE-SECTIONS; MONOCLONAL-ANTIBODY; WHOLE-BLOOD; POSTNATAL NEOVASCULARIZATION; PERIPHERAL-BLOOD; MARKERS; VASCULOGENESIS; ANGIOGENESIS; ENGRAFTMENT;
Keywords:
endothelial progenitor cells; allogeneic bone marrow transplantation chimerism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Brandt, SJ Vanderbilt Univ, Med Ctr, Div Hematol Oncol, Bone Marrow Transplant Program, Room 547 MRB 2, Nashville, TN 37232 USA Vanderbilt Univ Room 547 MRB 2 Nashville TN USA 37232 37232 USA
Citazione:
C. Ikpeazu et al., "Donor origin of circulating endothelial progenitors after allogeneic bone marrow transplantation", BIOL BLOOD, 6(3A), 2000, pp. 301-308

Abstract

Endothelial cell precursors circulate in blood and express antigens found on hematopoietic stem cells, suggesting that such precursors might be subject to transplantation. To investigate, we obtained adherence-depleted peripheral blood mononuclear cells from 3 individuals who had received a sex-mismatched allogeneic bone marrow transplant (BMT) and cultured the cells on fibronectin-coated plates with endothelial growth factors. The phenotype of the spindle-shaped cells that emerged in culture was characterized by immunofluorescent staining, and the origin of the cells was determined using a polymerase chain reaction (PCR)-based assay for polymorphic short tandem repeats (STRs). The cells manifested a number of endothelial characteristics-such as von Willebrand factor, CD31, and Flk-1/KDR expression; Bandeiraea simplicifolia lectin 1 binding; and acetylated low-density lipoprotein uptake-but lacked expression of certain markers of activation or differentiation,including intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and the epitope for the anti-endothelial cell antibody P1H12. For each patient and at all time points studied (ranging from 5 to 52 months aftertransplantation), STR-PCR analysis showed that cultured cells and nucleated blood cells came exclusively from the bone marrow donor. These results demonstrate that circulating endothelial progenitors are both transplantable and capable of long-term repopulation of human allogeneic BMT recipients.

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Documento generato il 27/11/20 alle ore 12:46:00