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Titolo:
The human multidrug resistance gene (MDR-1): Immunocytochemical detection of its expression in oral SCC
Autore:
Lo Muzio, L; Staibano, S; Pannone, G; Mignogna, MD; Serpico, R; Rubini, C; Fioroni, M; Fanali, S; Piattelli, A;
Indirizzi:
Univ Bari, Dept Dent & Surg, Bari, Italy Univ Bari Bari ItalyUniv Bari, Dept Dent & Surg, Bari, Italy Univ Naples Federico II, Dept Biomorphol & Funct Sci, Pathol Unit, Naples,Italy Univ Naples Federico II Naples Italy nct Sci, Pathol Unit, Naples,Italy Univ Naples Federico II, Dept Dent Sci, Naples, Italy Univ Naples FedericoII Naples Italy o II, Dept Dent Sci, Naples, Italy Univ Ancona, Inst Pathol Anat & Histopathol, Ancona, Italy Univ Ancona Ancona Italy Inst Pathol Anat & Histopathol, Ancona, Italy Univ Ancona, Inst Dent Sci, Ancona, Italy Univ Ancona Ancona ItalyUniv Ancona, Inst Dent Sci, Ancona, Italy Univ Chieti, Dept Dent Sci, Chieti, Italy Univ Chieti Chieti ItalyUniv Chieti, Dept Dent Sci, Chieti, Italy
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 5A, volume: 20, anno: 2000,
pagine: 2891 - 2897
SICI:
0250-7005(200009/10)20:5A<2891:THMRG(>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
P-GLYCOPROTEIN EXPRESSION; SQUAMOUS-CELL CARCINOMA; MULTIPLE-DRUG RESISTANCE; ACUTE MYELOID-LEUKEMIA; COLORECTAL CARCINOMAS; PROGNOSTIC FACTORS; TOPOISOMERASE-II; LUNG-CANCER; PROTEIN; OVEREXPRESSION;
Keywords:
chemotherapy; MDR-1; multidrug resistance; oral SCC; P-glycoprotein; P-170;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Lo Muzio, L Via Carelli 28, I-71100 Foggia, Italy Via Carelli 28 Foggia Italy I-71100 8, I-71100 Foggia, Italy
Citazione:
L. Lo Muzio et al., "The human multidrug resistance gene (MDR-1): Immunocytochemical detection of its expression in oral SCC", ANTICANC R, 20(5A), 2000, pp. 2891-2897

Abstract

A large number of oral cancer patients show poor or partial response to chemotherapy and the mechanisms ale poorly understood. At present, an MDR-I product, the P-170 glycoprotein, is the best known of the P-170 family and is involved in resistance to natural product-based chemotherapeutics, including taxanes, anthracyclines, vinca alkaloids, podophyllotoxins and camptothecins. Although several reports suggest that P-170 is clinically relevant in haematological malignancies, its role in solid tumours is not well understood. Its overexpression has been found to be correlated with the poor outcome observed in patients treated with chemotherapy and presenting drug I resistance. The aim of this study was to detect the protein expression patterns of MDR-I product by immunohistochemistry in formalin-fixed-paraffin-embedded tissues. For these reasons, 30 oral SCC and 6 healthy oral mucosa specimens were tested with anti-P-170 antibodies using standard streptavidin-biotin-peroxide technique. Immunohistochemistry demonstrated that 4 cases (66.6%) of normal oral mucosa and 24 cases (80%) of oral SCC showed positivity. Four cases (13.4%) showed strong positivity in tumour areas and complete negativity in normal epithelial cells adjacent to the tumour. No staining was observed in stromal structures, with the exception of the lymphocytic compartment that showed a strong staining as reported in literature for CD56and CD8+ cells. Four GI tumours (33%) and 2 G3 tumour (33%) showed strong positivity in areas with a higher degree of differentiation. P-170 positivity in normal epithelial cells of smoker patients, in differentiated area ofneoplasia and negativity or zonal positivity in undifferentiated area of tumour suggested that activation of the MDR-I gene or selection of intrinsically multidrug resistance neoplastic cells may occur at early stages of tumorigenesis of oral cancers, before the real evidence of cellular transformation. Thus the contact with possible chemical carcinogens, such as those oftobacco smoke, may induce activation of MDR-I gene. This study was conducted only on untreated carcinomas so for this reason it cannot indicate the real incidence of acquired multidrug resistance. The data of MDR-I product expression by immunohistochemistry in oral SCC might suggest that an overexpression of this protein could constitute a hallmark of potential more aggressive phenotype for this type of neoplasia and a rapid method for pre-screening tumours for a constitutive multidrug resistance in order to orientate the cancer treatment.

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Documento generato il 03/06/20 alle ore 07:44:22