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Titolo:
SOD-1 expression in pig coronary arterioles is increased by exercise training
Autore:
Rush, JWE; Laughlin, MH; Woodman, CR; Price, EM;
Indirizzi:
Univ Missouri, Dept Vet Biomed Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 et Biomed Sci, Columbia, MO 65211 USA Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 ovasc Res Ctr, Columbia, MO 65211 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 5, volume: 279, anno: 2000,
pagine: H2068 - H2076
SICI:
0363-6135(200011)279:5<H2068:SEIPCA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CU/ZN SUPEROXIDE-DISMUTASE; NITRIC-OXIDE; OXIDATIVE STRESS; RELAXING FACTOR; TRANSPORT CAPACITY; MESSENGER-RNA; FREE-RADICALS; SHEAR-STRESS; GENE; DOGS;
Keywords:
superoxide dismutase; microcirculation; endothelium; antioxidant enzymes; nitric oxide; endothelium-dependent dilation; oxidative stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Rush, JWE Univ Waterloo, Dept Kinesiol, BMH 1114, Waterloo, ON N2L 3G1, Canada Univ Waterloo BMH 1114 Waterloo ON Canada N2L 3G1 2L 3G1, Canada
Citazione:
J.W.E. Rush et al., "SOD-1 expression in pig coronary arterioles is increased by exercise training", AM J P-HEAR, 279(5), 2000, pp. H2068-H2076

Abstract

Coronary arterioles of exercise-trained (EX) pigs have enhanced nitric oxide (NO .)-dependent dilation. Evidence suggests that the biological half-life of NO . depends in part on the management of the superoxide anion. The purpose of this study was to test the hypothesis that expression of cytosolic copper/zinc-dependent superoxide dismutase (SOD)-1 is increased in coronary arterioles as a result of exercise training. Male Yucatan pigs either remained sedentary (SED, n = 4) or were EX (n = 4) on a motorized treadmill for 16-20 wk. Individual coronary arterioles (similar to 100-mum unpressurized internal diameter) were dissected and frozen. Coronary arteriole SOD-1 protein (via immunoblots) increased as a result of exercise training (2.16 +/- 0.35 times SED levels) as did SOD-1 enzyme activity (measured via inhibition of pyrogallol autooxidation; similar to 75% increase vs. SED). In addition, SOD-1 mRNA levels (measured via RT-PCR) were higher in EX arterioles (1.68 +/- 0.16 times the SED levels). There were no effects of exercise training on the levels of SOD-2 (mitochondrial), catalase, or p67(phox) proteins. Thus chronic aerobic exercise training selectively increases the levelsof SOD-1 mRNA, protein, and enzymatic activity in porcine coronary arterioles. Increased SOD-1 could contribute to the enhanced NO . -dependent dilation previously observed in EX porcine coronary arterioles by improving management of superoxide in the vascular cell environment, thus prolonging the biological half-life of NO ..

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Documento generato il 30/03/20 alle ore 19:50:06