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Titolo:
Gene transfer of recombinant endothelial nitric oxide synthase to liver invivo and in vitro
Autore:
Shah, V; Chen, AF; Cao, S; Hendrickson, H; Weiler, D; Smith, L; Yao, J; Katusic, ZS;
Indirizzi:
Mayo Clin & Mayo Fdn, Gastrointestinal Res Unit, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Unit, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Anesthesia Res Unit, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Unit, Rochester, MN 55905 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 5, volume: 279, anno: 2000,
pagine: G1023 - G1030
SICI:
0193-1857(200011)279:5<G1023:GTOREN>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CIRRHOTIC RAT-LIVER; PORTAL-HYPERTENSION; HEPATIC SINUSOIDS; CELLS; CONTRACTILITY; RESISTANCE; DYSFUNCTION; EXPRESSION; RECEPTOR; INJURY;
Keywords:
hepatic perfusion; adenovirus vector; beta-galactosidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Shah, V Mayo Clin & Mayo Fdn, Gastrointestinal Res Unit, 200 1st St SW,Alfred 2-435, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW,Alfred 2-435 Rochester MN USA 55905
Citazione:
V. Shah et al., "Gene transfer of recombinant endothelial nitric oxide synthase to liver invivo and in vitro", AM J P-GAST, 279(5), 2000, pp. G1023-G1030

Abstract

Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) contributes to hepatic vascular homeostasis. The aim of this study was to examine whether delivery of an adenoviral vector encoding eNOS gene to liver affects vasomotor function in vivo and the mechanism of NO production in vitro. Rats were administered adenoviruses encoding beta -galactosidase (AdCMVLacZ)or eNOS (AdCMVeNOS) via tail vein injection and studied 1 wk later. In animals transduced with AdCMVLacZ, beta -galactosidase activity was increased in the liver, most prominently in hepatocytes. In AdCMVeNOS-transduced animals, eNOS protein levels and catalytic activity were significantly increased. Overexpression of eNOS diminished baseline perfusion pressure and constriction in response to the alpha (1)-agonist methoxamine in the perfused liver. Transduction of cultured hepatocytes with AdCMVeNOS resulted in the targeting of recombinant eNOS to a perinuclear distribution and binding with the NOS-activating protein heat shock protein 90. These events were associated with increased ionomycin-stimulated NO release. In summary, this is the first study to demonstrate successful delivery of the recombinant eNOS geneto liver in vivo and in vitro with ensuing NO production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:09:58