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Titolo:
SPECT tracer [I-123]IBZM has similar affinity to dopamine D2 and D3 receptors
Autore:
Videbaek, C; Toska, K; Scheideler, MA; Paulson, OB; Knudsen, GM;
Indirizzi:
Copenhagen Univ Hosp, Rigshosp, Neurobiol Res Unit 9201, Copenhagen O, Denmark Copenhagen Univ Hosp Copenhagen Denmark O it 9201, Copenhagen O, Denmark Novo Nordisk, Malov, Denmark Novo Nordisk Malov DenmarkNovo Nordisk, Malov, Denmark
Titolo Testata:
SYNAPSE
fascicolo: 3, volume: 38, anno: 2000,
pagine: 338 - 342
SICI:
0887-4476(200012)38:3<338:ST[HSA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON EMISSION TOMOGRAPHY; HUMAN BRAIN; D-3 RECEPTOR; IBZM-SPECT; I-123 IBZM; SCHIZOPHRENIC-PATIENTS; PARKINSONS-DISEASE; BINDING-PROPERTIES; D4 RECEPTORS; RAT;
Keywords:
[I-123]IBZM; SPECT; dopamine receptors; in vitro;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Videbaek, C Austr 74, CH-4051 Basel, Switzerland Austr 74 Basel Switzerland CH-4051 H-4051 Basel, Switzerland
Citazione:
C. Videbaek et al., "SPECT tracer [I-123]IBZM has similar affinity to dopamine D2 and D3 receptors", SYNAPSE, 38(3), 2000, pp. 338-342

Abstract

Emission tomography investigations of the pathophysiological involvement of the cerebral dopaminergic transmitter system in the living human brain relies heavily on a careful selection of the most suitable radioligand. In recent years, many clinical studies have employed [I-123]IBZM in SPECT studies. The aim of the present study was to characterize the binding of IBZM to dopaminergic receptor subtypes as a means of elucidating which receptor subtypes are visualized and examined by [I-123]IBZM. The affinity of IBZM for each of the major human dopamine receptors (D1, D2(short), D3, D4(4.2), andD5 receptor) was determined by competitive radioligand binding assay usingmembranes prepared from clonal cell lines expressing the different subtypes. Radioligands with high affinity for the D1, and D5 receptors ([H-3]SCH-23390), dopamine D2(short) and D4(4.2) receptors ([H-3]Spiroperidol), and dopamine D3 receptor ([H-3]7-OH-DPAT) were used to measure specific binding. Corresponding unlabeled displacing ligands for determination of nonspecificbinding were employed. Assays were performed at 25 degreesC. These experiments show that for IBZM K-i values were 1.6 nM for dopamine D2(s) receptorsand 2.2 nM for dopamine D3 receptors. There was no binding of IBZM to D1(A), D5, or D4(4.2) receptors. In conclusion, when [I-123]IBZM is used as SPECT tracer, the studies reflect dopaminergic D2 as well as D3 receptor binding. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 06:06:06