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Titolo:
Inducible nitric oxide synthase knockout mouse macrophages disclose prooxidant effect of interferon-gamma on low-density lipoprotein oxidation
Autore:
Niu, XL; Xia, Y; Hoshiai, K; Tanaka, K; Sawamura, S; Nakazawa, H;
Indirizzi:
Tokai Univ, Sch Med, Dept Physiol, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Dept Microbiol, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan
Titolo Testata:
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
fascicolo: 4, volume: 4, anno: 2000,
pagine: 363 - 371
SICI:
1089-8603(200008)4:4<363:INOSKM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCAVENGER RECEPTOR EXPRESSION; MONOCYTE-DERIVED MACROPHAGES; TUMOR-NECROSIS-FACTOR; MEDIATED OXIDATION; LDL OXIDATION; INHIBITION; ATHEROSCLEROSIS; CELLS; NEUTROPHILS; LYMPHOCYTES;
Keywords:
low-density lipoprotein; oxidation; IFN-gamma; nitric oxide; iNOS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Nakazawa, H Tokai Univ, Sch Med, Dept Physiol, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 gawa 2591193, Japan
Citazione:
X.L. Niu et al., "Inducible nitric oxide synthase knockout mouse macrophages disclose prooxidant effect of interferon-gamma on low-density lipoprotein oxidation", NITRIC OXID, 4(4), 2000, pp. 363-371

Abstract

To test our hypothesis that interferon-gamma (IFN-gamma) has a direct prooxidant effect on macrophage-mediated LDL oxidation behind its antioxidant effect via induction of inducible nitric oxide synthase (iNOS), we incubatedLDL with wild-type (iNOS(+/+)) or iNOS knockout mouse (iNOS(-/-)) macrophages preincubated with IFN-gamma or IFN-gamma plus lipopolysaccharide (IFN-gamma /LPS) for 24 h, LDL oxidation was measured in terms of formation of thiobarbituric acid reactive substances (TBARS) and electrophoretic mobility. Thiol production, nitrite production, and superoxide production from macrophages were measured by using Ellman's assay, the Griess reagent, and the SOD-inhibitable cytochrome c reduction method, respectively, IFN-gamma aloneor combined with LPS induced iNOS expression and increased nitrite production in iNOS(+/+) macrophages, but not in iNOS(-/-) macrophages, TEARS formation from LDL was suppressed in IFN-gamma- and IFN-gamma /LPS-treated iNOS(/+) macrophages but was increased in IFN-gamma -treated iNOS(-/-) macrophages. In the presence of N-G-monomethyl-L-arginine (L-NMMA), a NOS inhibitor, the suppressive effect of IFN-gamma and IFN-gamma /LPS was abolished and TEARS formation was even increased to a level above that of untreated iNOS(/+) macrophage, NOC 18, an NO donor, dose dependently inhibited macrophage-mediated LDL oxidation. IFN-gamma increased superoxide and thiol productions in both types of macrophages. We conclude that IFN-gamma promotes macrophage-mediated LDL oxidation by stimulating superoxide and thiol production under conditions where iNOS-catalyzed NO release is restricted. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:09:08