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Titolo:
Regulation of the Pcl7-Pho85 cyclin-cdk complex by Pho81
Autore:
Lee, M; Oregan, S; Moreau, JL; Johnson, AL; Johnston, LH; Goding, CR;
Indirizzi:
Marie Curie Res Inst, Eukaryot Transcript Lab, Surrey RH8 OTL, England Marie Curie Res Inst Surrey England RH8 OTL Lab, Surrey RH8 OTL, England Natl Inst Med Res, Div Yeast Genet, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA Genet, London NW7 1AA, England
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 2, volume: 38, anno: 2000,
pagine: 411 - 422
SICI:
0950-382X(200010)38:2<411:ROTPCC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT PROTEIN-KINASE; RNA-POLYMERASE-II; SACCHAROMYCES-CEREVISIAE; CELL-CYCLE; BUDDING YEAST; TRANSCRIPTIONAL CONTROL; NEGATIVE REGULATORS; GENE; PHOSPHORYLATION; PHO80-PHO85;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Goding, CR Marie Curie Res Inst, Eukaryot Transcript Lab, Surrey RH8 OTL, England Marie Curie Res Inst Surrey England RH8 OTL RH8 OTL, England
Citazione:
M. Lee et al., "Regulation of the Pcl7-Pho85 cyclin-cdk complex by Pho81", MOL MICROB, 38(2), 2000, pp. 411-422

Abstract

Saccharomyces cerevisiae strains lacking a functional Pho85 cyclin-dependent kinase (cdk) exhibit a complex phenotype, including deregulation of phosphatase genes controlled by the transcription factor Pho4, slow growth on rich media, failure to grow using galactose, lactate or glycerol as a carbonsource and hyperaccumulation of glycogen. The ability of Pho85 to regulatethe transcription factor Pho4 is mediated by its association the Pho80 cyclin. Some other regulatory functions of the Pho85 cdk have been shown to bemediated via its interaction with a recently identified family of Pho80-related cyclins (Pcls). Here, we show that the poorly characterized Pho80-like protein Pcl7 forms a functional kinase complex with the Pho85 cdk, and that the activity of this complex is inhibited in response to phosphate starvation. Additionally, we show that Pcl7 interacts with the phosphate-regulated cyclin-cdk inhibitor Pho81, and that the regulation of the Pcl7-Pho85 complex in response to changes in phosphate levels is dependent on Pho81. Thus, we demonstrate for the first time that the Pho81 regulator is not dedicated to regulating Pho80, but may act to co-ordinate the activity of both the Pho80-Pho85 and Pcl7-Pho85 cyclin-cdk complexes in response to phosphate levels. We also demonstrate that expression of Pcl7 is cell cycle regulated, with maximal activity occurring in mid to late S-phase, perhaps suggesting a role for Pcl7 in cell cycle progression. Finally, we describe the phenotype of pcl7 Delta and pcl6 Delta yeast strains that have defects in carbonsource utilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 22:02:46