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Titolo:
migA, a quorum-responsive gene of Pseudomonas aeruginosa, is highly expressed in the cystic fibrosis lung environment and modifies low-molecular-masslipopolysaccharide
Autore:
Yang, HJ; Matewish, M; Loubens, I; Storey, DG; Lam, JS; Jin, SG;
Indirizzi:
Univ Florida, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 icrobiol, Gainesville, FL 32610 USA Univ Guelph, Dept Microbiol, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 icrobiol, Guelph, ON N1G 2W1, Canada Univ Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada Univ Calgary Calgary AB Canada T2N 1N4 l Sci, Calgary, AB T2N 1N4, Canada
Titolo Testata:
MICROBIOLOGY-UK
, volume: 146, anno: 2000,
parte:, 10
pagine: 2509 - 2519
SICI:
1350-0872(200010)146:<2509:MAQGOP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHAMNOLIPID BIOSURFACTANT SYNTHESIS; POLYACRYLAMIDE GELS; ESCHERICHIA-COLI; NUCLEOTIDE-SEQUENCE; VIRULENCE GENES; SILVER STAIN; OUTER-CORE; LASA; STRAINS; TRANSCRIPTION;
Keywords:
migA; mucus; LPS and quorum response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Jin, SG Univ Florida, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 , Gainesville, FL 32610 USA
Citazione:
H.J. Yang et al., "migA, a quorum-responsive gene of Pseudomonas aeruginosa, is highly expressed in the cystic fibrosis lung environment and modifies low-molecular-masslipopolysaccharide", MICROBIO-UK, 146, 2000, pp. 2509-2519

Abstract

Pseudomonas aeruginosa is an opportunistic human pathogen which poses a major threat to patients with cystic fibrosis (CF). Excessive amounts of mucus present in the lungs of CF patients promotes the colonization of P. aeruginosa, The migA gene, encoding a putative glycosyltransferase, has been shown to be highly inducible by respiratory mucus derived from CF patients. Inthis study, it is further demonstrated by population transcript analysis that the migA gene is highly expressed in the CF lung environment. Deletion analysis of the migA promoter identified a las-box-like sequence commonly found in promoters that are responsive to quorum sensing regulation. Furtheranalysis of migA expression in quorum-sensing-defective strains, as well as its expression in response to autoinducer molecules, demonstrated that migA is regulated by the RhII/RhIR quorum sensing regulatory system. Functionally, as the MigA sequence homology data suggested, the migA gene indeed affects the structure of LPS in P, aeruginosa. Increased expression of the migA gene results in a loss of core-plus-one LPS, while having no obvious effect on the long-chain O-antigen-bearing LPS. Although the exact biological role of the core-plus-one LPS is not clear, these experimental results suggest that migA up-regulation in the CF lung environment is part of the adaptive response which confers on P, aeruginosa a survival advantage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 12:22:43