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Titolo:
POLYAMINE-DEPENDENT ALTERATIONS IN THE STRUCTURE OF MICROFILAMENTS, GOLGI-APPARATUS, ENDOPLASMIC-RETICULUM, AND PROTEOGLYCAN SYNTHESIS IN BHK CELLS
Autore:
PARKKINEN JJ; LAMMI MJ; AGREN U; TAMMI M; KEINANEN TA; HYVONEN T; ELORANTA TO;
Indirizzi:
UNIV KUOPIO,DEPT BIOCHEM & BIOTECHNOL,POB 1627 FIN-70211 KUOPIO FINLAND UNIV KUOPIO,DEPT BIOCHEM & BIOTECHNOL FIN-70211 KUOPIO FINLAND UNIV KUOPIO,DEPT PATHOL FIN-70211 KUOPIO FINLAND UNIV KUOPIO,DEPT ANAT FIN-70211 KUOPIO FINLAND
Titolo Testata:
Journal of cellular biochemistry
fascicolo: 2, volume: 66, anno: 1997,
pagine: 165 - 174
SICI:
0730-2312(1997)66:2<165:PAITSO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE CHONDROCYTES; HAMSTER-KIDNEY CELLS; ORNITHINE DECARBOXYLASE; CHONDROITIN SULFATE; PROTEIN-DEGRADATION; MESSENGER-RNA; BREFELDIN-A; ACTIN; PROLIFERATION; CYTOSKELETON;
Keywords:
CELL SHAPE; CYTOSKELETON; STRESS FIBERS; AUTOPHAGY; VACUOLAR DEGRADATION; HYALURONAN; CHONDROITIN SULFATE; KERATAN SULFATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Parkkinen et al., "POLYAMINE-DEPENDENT ALTERATIONS IN THE STRUCTURE OF MICROFILAMENTS, GOLGI-APPARATUS, ENDOPLASMIC-RETICULUM, AND PROTEOGLYCAN SYNTHESIS IN BHK CELLS", Journal of cellular biochemistry, 66(2), 1997, pp. 165-174

Abstract

The activity of ornithine decarboxylase, the key enzyme in the synthesis of polyamines, is essential for proliferation and differentiation of all living cells. Two inhibitors of ornithine decarboxylase, alpha-difluoromethylornithine (DFMO) and 1-aminooxy-3-aminopropane (APA), caused swelling of endoplasmic reticulum (ER) and medial and trans Golgicisternae, and the disappearance of stress fibers, as visualized by staining with fluorescent concanavalin A (ConA), CG-NBD-ceramide or wheat germ agglutinin (WCA), and phalloidin, respectively. In contrast, the pattern of microtubules, stained with a beta-tubulin antibody, was not affected. Rough ER seemed to be especially affected in polyamine deprivation forming whorls and involutions, which were observed by transmission electron microscopy. Since ER and Golgi apparatus are vital parts of the glycosylation and secretory machinery of the cell, we tested the ability of these structurally altered cell organelles to synthesize proteoglycans using [H-3]glucosamine and [S-35]sulfate as precursors. The total incorporation rate into proteoglycans and hyaluronan was not reduced in polyamine-deprived cells, suggesting that the total glycosylation capacity of cells was not affected. However, the synthesis of a high molecular weight proteoglycan containing chondroitin and keratan sulfate was completely inhibited. The remodeling of cytoskeleton and rough endoplasmic reticulum in polyamine deprivation may perturbthe synthesis and secretion of the components of membrane skeleton and of the extracellular matrix, e.g., proteoglycans. Rough ER and cytoskeleton may be the targets where polyamines affect cell proliferation and differentiation. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 10:34:04