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Titolo:
Ultrastructure of sea urchin calcified tissues after high-pressure freezing and freeze substitution
Autore:
Ameye, L; Hermann, R; Dubois, P;
Indirizzi:
Free Univ Brussels, Biol Marine Lab, B-1050 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1050 Lab, B-1050 Brussels, Belgium ETH Zurich, Inst Biochem, Electron Microscopy Lab 1, CH-8092 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8092 Lab 1, CH-8092 Zurich, Switzerland
Titolo Testata:
JOURNAL OF STRUCTURAL BIOLOGY
fascicolo: 2, volume: 131, anno: 2000,
pagine: 116 - 125
SICI:
1047-8477(200008)131:2<116:UOSUCT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY MESENCHYME CELLS; ELECTRON-MICROSCOPY; ARTICULAR-CARTILAGE; CHEMICAL FIXATION; DESIGN STRATEGIES; SPICULE FORMATION; MATRIX; ECHINODERMATA; PRESERVATION; BIOMINERALIZATION;
Keywords:
mineralized tissues; biomineralization; freeze substitution; gallic acid; high-pressure freezing muscle; organic matrix; Paracentrotus lividus; pedicellaria; skeleton; tooth; ultrastructure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Dubois, P Free Univ Brussels, Biol Marine Lab, CP 160-15,50 Av FD Roosevelt, B-1050 Brussels, Belgium Free Univ Brussels CP 160-15,50 Av FD RooseveltBrussels Belgium B-1050
Citazione:
L. Ameye et al., "Ultrastructure of sea urchin calcified tissues after high-pressure freezing and freeze substitution", J STRUCT B, 131(2), 2000, pp. 116-125

Abstract

The improvements brought by high-pressure freezing/freeze substitution fixation methods to the ultrastructural preservation of echinoderm mineralizedtissues are investigated in developing pedicellariae and teeth of the echinoid Paracentrotus lividus. Three freeze substitution (FS) protocols were tested: one in the presence of osmium tetroxide, one in the presence of uranyl acetate, and the last in the presence of gallic acid. FS in the presenceof osmium tetroxide significantly improved cell ultrastructure preservation and should especially be used for ultrastructural studies involving vesicles and the Golgi apparatus. With all protocols, multivesicular bodies, suggested to contain Ca2+, were evident for the first time in skeleton-formingcells. FS in the presence of gallic acid allowed us to confirm the structured and insoluble character of a part of the organic matrix of mineralization in the calcification sites of the tooth, an observation which modifies the current understanding of biomineralization control in echinoderms. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:32:27