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Titolo:
Phenylalanine ammonia-lyase genes involved in anthocyanin synthesis and the regulation of its expression in suspension cultured carrot cells
Autore:
Ozeki, Y; Ito, Y; Sasaki, N; Oyanagi, M; Akimoto, H; Chikagawa, Y; Takeda, J;
Indirizzi:
Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan Tokyo Univ Agr & Technol Koganei Tokyo Japan 1848588 Tokyo 1848588, Japan Kyoto Univ, Fac Agr, Dept Agr Chem, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Agr Chem, Sakyo Ku, Kyoto 6068502, Japan
Titolo Testata:
JOURNAL OF PLANT RESEARCH
fascicolo: 1111, volume: 113, anno: 2000,
pagine: 319 - 326
SICI:
0918-9440(200009)113:1111<319:PAGIIA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHALCONE SYNTHASE GENE; CIS-ACTING ELEMENTS; DNA-BINDING; TRANSCRIPTIONAL ACTIVATORS; TISSUE-CULTURES; LIGASE GENES; PROTEIN; METHYLATION; INDUCTION; ELICITOR;
Keywords:
anthocyanin; carrot; differentiation; myb; phenylalanine ammonia-lyase (PAL);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Ozeki, Y Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan Tokyo Univ Agr & Technol Koganei Tokyo Japan 1848588 8588, Japan
Citazione:
Y. Ozeki et al., "Phenylalanine ammonia-lyase genes involved in anthocyanin synthesis and the regulation of its expression in suspension cultured carrot cells", J PLANT RES, 113(1111), 2000, pp. 319-326

Abstract

Phenylalanine ammonia-lyase (PAL) catalyses the first step in the phenylpropanoid pathway and is induced during differentiation and by various stimuli. PAL activity is slowly induced during anthocyanin synthesis which occursin a medium lacking 2,4-D and is also induced rapidly and transiently by transfer of cells to fresh medium and lowering the cell density. From the carrot genomic library, four clones of PAL genes, gDcPAL1, 2, 3 and 4, were obtained. Analyses of nucleotide sequences revealed that only the gDcPAL3 gene is responsible for the induction of anthocyanin synthesis by 2, 4-D, Several cis-elements, boxes M, P, A, L, and G, exist in the proximal promoter region of gDcPAL8 Transient expression experiments in carrot protoplasts using deletion mutants of the proximal promoter region of gDcPAL3 gene showedthat boxes M and L, both of which contain core sequences of the Myb binding sites, might play an important role in gDcPAL3 promoter activity, Four myb cDNAs, Dcmyb8, 10, 12 and 14 were obtained from a carrot subtracted cDNA library and their structure and expression patterns were analyzed. In addition to the analysis of the proximal region of gDcPAL3 promoter, the possibility of the regulation of gene expression by genomic DNA structure and chromatin modification in metabolic differentiation is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:07:31