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Titolo:
Transport mechanisms of 3-[I-123]Iodo-alpha-methyl-L-tyrosine in a human glioma cell line: Comparison with [H-3-methyl]-L-methionine
Autore:
Langen, KJ; Muhlensiepen, H; Holschbach, M; Hautzel, H; Jansen, P; Coenen, HH;
Indirizzi:
Res Ctr Julich GMBH, Inst Med, D-52425 Julich, Germany Res Ctr Julich GMBH Julich Germany D-52425 Med, D-52425 Julich, Germany Res Ctr Julich GMBH, Inst Nucl Chem, D-52425 Julich, Germany Res Ctr Julich GMBH Julich Germany D-52425 Chem, D-52425 Julich, Germany Res Ctr Julich GMBH, Cent Inst Appl Math, D-52425 Julich, Germany Res Ctr Julich GMBH Julich Germany D-52425 Math, D-52425 Julich, Germany Univ Dusseldorf, Clin Nucl Med, D-4000 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-4000 ed, D-4000 Dusseldorf, Germany
Titolo Testata:
JOURNAL OF NUCLEAR MEDICINE
fascicolo: 7, volume: 41, anno: 2000,
pagine: 1250 - 1255
SICI:
0161-5505(200007)41:7<1250:TMO3IA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; IODINE-123-ALPHA-METHYL TYROSINE; BRAIN-TUMORS; L-3-IODO-ALPHA-METHYL TYROSINE; PROTEIN-SYNTHESIS; METHIONINE UPTAKE; GRADE GLIOMAS; IN-VIVO; PET; SPECT;
Keywords:
amino acid transport; 3-[I-123]iodo-alpha-methyl-L-tyrosine; [H-3-methyl]-L-methionine; glioma cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Langen, KJ Res Ctr Julich GMBH, Inst Med, D-52425 Julich, Germany Res Ctr Julich GMBH Julich Germany D-52425 25 Julich, Germany
Citazione:
K.J. Langen et al., "Transport mechanisms of 3-[I-123]Iodo-alpha-methyl-L-tyrosine in a human glioma cell line: Comparison with [H-3-methyl]-L-methionine", J NUCL MED, 41(7), 2000, pp. 1250-1255

Abstract

The amino acid analog 3-[I-123]iodo-oc-methyl-L-tyrosine (IMT) is under clinical evaluation as a SPECT tracer of amino acid transport in brain tumors. This study investigated the carrier systems involved in IMT transport in human glioma cells in comparison with [H-3-methyl]-L-methionine (H-3-MET). Methods: Human glioma cells, type 86HG-39, were cultured and incubated for 1 min at 37 degrees C with IMT and H-3-MET in the lag phase (1.2 d after seeding), exponential growth phase (3 d after seeding), and plateau phase (8 d after seeding). Experiments were performed in the presence and absence ofNa+, during inhibition of system L amino acid transport by 2-aminobicyclo[2.2.1]heptane-e-carboxylic acid (BCH), and during inhibition of system A amino acid transport by 2-(methylamino)-isobutyric acid (MeAIB). Results: IMTand H-3-MET uptake decreased by 55%-73% when the cells entered from the exponential growth phase into the plateau phase (P < 0.05; n = 3-11). Inhibition by BCH reduced uptake of IMT in the lag phase, exponential growth phase, and plateau phase by 90%-98% (P < 0.001; n = 3-6) and the uptake of H-3-MET by 73%-83% (P < 0.001; n = 3-11). In a Na+-free medium H-3-MET uptake was reduced by 23%-33% (P < 0.05; n = 3-11), whereas IMT uptake was not significantly different. MeAIB showed no significant effect on IMT or H-3-MET uptake in either phase. Conclusion: Transport of both IMT and H-3-MET dependson the proliferation rate of human glioma cells in vitro and is dominated by BCH-sensitive transport. These data indicate that system L is induced inrapidly proliferating glioma cells and is the main contributor to the uptake of both tracers. H-3-MET transport showed a minor Na+ dependency that was not attributable to system A. The similarity of transport mechanisms of both tracers emphasizes the clinical equivalence of IMT SPECT and C-11-MET PET for the diagnostic evaluation of gliomas.

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Documento generato il 24/01/21 alle ore 05:23:41