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Titolo:
cDNA cloning of biologically active chicken interleukin-18
Autore:
Schneider, K; Puehler, F; Baeuerle, D; Elvers, S; Staeheli, P; Kaspers, B; Weining, KC;
Indirizzi:
Univ Freiburg, Dept Virol, Inst Med Mikrobiol & Hyg, Abt Virol, D-79008 Freiburg, Germany Univ Freiburg Freiburg Germany D-79008 Virol, D-79008 Freiburg, Germany Univ Munich, Inst Tierphysiol, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 Tierphysiol, D-80539 Munich, Germany
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 10, volume: 20, anno: 2000,
pagine: 879 - 883
SICI:
1079-9907(200010)20:10<879:CCOBAC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-INDUCING FACTOR; BLOOD MONONUCLEAR-CELLS; INTERFERON-GAMMA; IFN-GAMMA; BINDING-PROTEIN; EXPRESSION; RECEPTOR; CYTOKINE; IL-18; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Staeheli, P Univ Freiburg, Dept Virol, Inst Med Mikrobiol & Hyg, Abt Virol, Hermann Herder Str 11, D-79008 Freiburg, Germany Univ Freiburg Hermann Herder Str 11 Freiburg Germany D-79008
Citazione:
K. Schneider et al., "cDNA cloning of biologically active chicken interleukin-18", J INTERF CY, 20(10), 2000, pp. 879-883

Abstract

By searching a chicken EST database, we identified a cDNA clone that appeared to contain the entire open reading frame (ORF) of chicken interleukin-18 (ChIL-18). The encoded protein consists of 198 amino acids and exhibits approximately 30% sequence identity to IL-18 of humans and various others mammals. Sequence comparisons reveals a putative caspase-1 cleavage site at aspartic acid 29 of the primary translation product, indicating that mature ChIL-18 might consist of 169 amino acids. Bacterially expressed ChIL-18 in which the N-terminal 29 amino acids of the putative precursor molecule werereplaced by a histidine tag induced the synthesis of interferon-gamma (IFN-gamma) in cultured primary chicken spleen cells, indicating that the recombinant protein is biologically active.

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Documento generato il 25/01/20 alle ore 16:41:35