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Titolo:
The role of tetrahydrobiopterin in the activation of oxygen by nitric-oxide synthase
Autore:
Bec, N; Gorren, AFC; Mayer, B; Schmidt, PP; Andersson, KK; Lange, R;
Indirizzi:
INSERM, U128, F-34293 Montpellier, France INSERM Montpellier France F-34293 ERM, U128, F-34293 Montpellier, France Karl Franzens Univ Graz, Inst Pharmakol & Toxikol, A-8010 Graz, Austria Karl Franzens Univ Graz Graz Austria A-8010 oxikol, A-8010 Graz, Austria Univ Oslo, Dept Biochem, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316 niv Oslo, Dept Biochem, N-0316 Oslo, Norway
Titolo Testata:
JOURNAL OF INORGANIC BIOCHEMISTRY
fascicolo: 3, volume: 81, anno: 2000,
pagine: 207 - 211
SICI:
0162-0134(20000831)81:3<207:TROTIT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-TEMPERATURE; L-ARGININE; MECHANISM; COMPLEX; CONVERSION; CITRULLINE; ISOFORMS; SYSTEM; PTERIN;
Keywords:
nitric-oxide synthase; tetrahydrobiopterin; trihydrobiopterin; low-temperature spectroscopy; electron paramagnetic resonance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Lange, R INSERM, U128, IFR 24, F-34293 Montpellier, France INSERM IFR 24 Montpellier France F-34293 293 Montpellier, France
Citazione:
N. Bec et al., "The role of tetrahydrobiopterin in the activation of oxygen by nitric-oxide synthase", J INORG BIO, 81(3), 2000, pp. 207-211

Abstract

We have studied the reaction of reduced nitric-oxide synthase (NOS) with molecular oxygen at -30 degrees C. In the first reaction cycle (from L-Arg to hydroxy-L-Arg), an oxygen adduct complex formed rapidly. Experiments in the absence of the reductase domain demonstrated that this complex was then further reduced by one electron stemming from the cofactor tetrahydrobiopterin (BH4). Spectral evidence suggested an iron(IV) porphyrin pi-cation radical as an intermediate. The nature of the oxidized BH4 was identified by EPR as a BH3(.) radical. Within the second cycle (from hydroxy-L-Arg to citrulline and NO), an iron(III)-NO complex could be identified clearly by its spectral characteristics. The strict requirement of BH4 for its formation suggests that BH4 plays a redox role, although transient, also in the second reaction cycle. (C) 2000 Elsevier Science S.A. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 07:39:24