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Titolo:
Efficient transformation of primary human amniocytes by E1 functions of Ad5: Generation of new cell lines for adenoviral vector production
Autore:
Schiedner, G; Hertel, S; Kochanek, S;
Indirizzi:
Univ Cologne, Ctr Mol Med, D-50931 Cologne, Germany Univ Cologne CologneGermany D-50931 r Mol Med, D-50931 Cologne, Germany
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 15, volume: 11, anno: 2000,
pagine: 2105 - 2116
SICI:
1043-0342(200010)11:15<2105:ETOPHA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; RECOMBINANT ADENOVIRUSES; E1-DELETED ADENOVIRUS; EARLY REGION-1; GENE-THERAPY; KB CELLS; EXPRESSION; CONSTRUCTION; PROTEINS; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Schiedner, G Univ Cologne, Ctr Mol Med, Kerpenerstr 34, D-50931 Cologne, Germany Univ Cologne Kerpenerstr 34 Cologne Germany D-50931 Germany
Citazione:
G. Schiedner et al., "Efficient transformation of primary human amniocytes by E1 functions of Ad5: Generation of new cell lines for adenoviral vector production", HUM GENE TH, 11(15), 2000, pp. 2105-2116

Abstract

Primary human cells are relatively refractory to transformation by adenoviral E1 functions. For almost two decades, human embryonic kidney (HEK)-derived 293 cells have been the only E1-complementing cell line suitable for production of E1-deleted adenoviral vectors. More recently, new vector production cell lines have been derived from human embryonic retina (HER) cells, a cell type that is difficult to obtain. We were surprised to find that readily available primary human amniocytes are efficiently transformed by adenoviral E1 functions. We selected cell lines that allow high-titer production of recombinant adenoviral vectors. The generation of replication-competent adenovirus (RCA) during production, caused by homologous recombination between vector and cellular DNA, was excluded by designing the transforming plasmid to lack sequence overlap with current adenoviral vectors. In addition, we generated an infectious plasmid that can be used for convenient generation of first-generation adenoviral vectors in Escherichia coli and that matches the E1 complementation in the new production cell lines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 01:20:48