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Titolo:
Trypanosoma cruzi: Cloning and characterization of a RAB7 gene
Autore:
Leal, ST; Araripe, JR; Urmenyi, TP; Cross, GAM; Rondinelli, E;
Indirizzi:
UFRJ, CCS, Inst Biofis Carlos Chagas Filho, BR-21949900 Rio De Janeiro, Brazil UFRJ Rio De Janeiro Brazil BR-21949900 BC21949900 Rio De Janeiro, Brazil Rockefeller Univ, New York, NY 10021 USA Rockefeller Univ New York NY USA10021 eller Univ, New York, NY 10021 USA
Titolo Testata:
EXPERIMENTAL PARASITOLOGY
fascicolo: 1, volume: 96, anno: 2000,
pagine: 23 - 31
SICI:
0014-4894(200009)96:1<23:TCCACO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING-PROTEINS; DICTYOSTELIUM-DISCOIDEUM; VESICULAR TRANSPORT; EPIMASTIGOTE FORMS; GTPASES; EXPRESSION; RAS; ENDOCYTOSIS; YEAST; IDENTIFICATION;
Keywords:
endocytosis; genomic organization; kinetoplastida; nucleotide sequence; RAB protein; RAB7; Ras-related gene; small GTP binding protein; Trypanosoma cruzi;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Rondinelli, E UFRJ, CCS, Inst Biofis Carlos Chagas Filho, Bloco G,Cidade Univ,Ilha Fundao, BR-21949900 Rio De Janeiro, Brazil UFRJ Bloco G,Cidade Univ,Ilha Fundao Rio De Janeiro Brazil BR-21949900 BC
Citazione:
S.T. Leal et al., "Trypanosoma cruzi: Cloning and characterization of a RAB7 gene", EXP PARASIT, 96(1), 2000, pp. 23-31

Abstract

The small monomeric GTP-binding proteins of the RAB subfamily are key regulatory elements of the machinery that controls membrane traffic in eukaryotic cells. These proteins have been localized to many different intracellular organelles, on both endocytic and exocytic compartments, suggesting that each step of vesicular traffic can involve a specific RAB protein. The presence of conserved amino acid domains in these proteins has allowed the cloning of their genes from several organisms, including yeast, plants, humans,and parasites. In this work we describe the identification, cloning, and characterization of a RAB gene homologue in Trypanosoma cruzi (TcRAB7). Our data indicate that this gene is present as a single copy in the T. cruzi genome, located on a 2.25-Mb chromosomal DNA. TcRAB7 is expressed in T. cruziepimastigotes, metacyclic trypomastigotes, and spheromastigotes. We established transformed cell lines that express two versions of an epitope-taggedTcRAB7 protein: one wild type (pTAG) and one deleted at the C-terminal cysteines (p Delta CXC). Wild-type TcRAB7 protein (pTAG) appears to be localized exclusively in the membrane fraction, while the mutated TcRAB7 protein (p Delta CXC) loses the ability to associate with the membrane, showing onlycytosolic localization. Also, we produced the recombinant TcRAB7 protein and demonstrated that it binds GTP. The identification of exo- and endocyticmachinery components in I: cruzi and their function would provide specificmarkers of these subcellular compartments, thereby unveiling important aspects of vesicular traffic in this parasite. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:41:20